产品号 #100-0209_C
Mouse monoclonal antibody against human, rhesus, cynomolgus CD81, unconjugated
概述
The 5A6 antibody reacts with an epitope located in the large extracellular loop (EC2) of human CD81 (TAPA-1). CD81 exhibits broad expression, including on the surface of B and T cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It is also a marker characteristic of extracellular vesicles.
CD81, a 26 kDa non-glycosylated type III transmembrane protein, belongs to the tetraspanin family. Tetraspanins contain four transmembrane domains, two extracellular loops, and short cytoplasmic N- and C-termini. CD81 associates with numerous integrins, co-receptors, and other proteins to form multimolecular complexes in the plasma membrane called tetraspanin-enriched microdomains. Palmitoylation of the juxtamembrane cysteine residues is important for its association with other proteins and the plasma membrane.
CD81 modulates a diverse array of biological functions including cell-cell adhesion and fusion, cell migration, and proliferation. For example, together with CD19 and CD21, CD81 forms a co-receptor for the B cell receptor on B cells. The epitope of the 5A6 antibody is hidden when CD81 is in complex with CD19 but accessible following activation of the B cell. Mutations in CD81 have been associated with the immune disorder common variable immunodeficiency (CVID). CD81 has also been implicated in mediating the entry of hepatitis C virus into cells.
Subtype
Primary Antibodies
Target Antigen
CD81
Alternative Names
CVID6
Reactive Species
African Green Monkey, Baboon, Chimpanzee, Cynomolgus, Human, Rhesus
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Other
Application
Flow Cytometry, Western Blotting
Area of Interest
Extracellular Vesicle Research
Clone
5A6
Gene ID
975
Isotype
IgG1, kappa
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (4)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
