产品号 #60105_C
Mouse monoclonal IgG2b antibody against human CD68
概述
The Y1/82A antibody reacts with human CD68, an ~110 kDa type 1 transmembrane glycoprotein and member of the sialomucin, LAMP and scavenger receptor families. The heavily glycosylated extracellular domain of CD68, which contains a praline-rich spacer region separating mucin and LAMP domains, binds low-density lipoprotein and certain lectins and selectins, though the function of CD68 remains unclear. CD68 is highly expressed in lysosomes, endosomes and cytoplasmic granules, and more weakly on the surface of macrophages, monocytes, neutrophils, basophils, dendritic cells and NK cells. It has also been detected in the cytoplasm of γ/δ T cells, LAK cells, fibroblasts, endothelial cells and subsets of B cells and hematopoietic progenitors, as well as in various non-hematopoietic tissues such as liver and kidney. The Y1/82A antibody recognizes an epitope distinct from those of antibody clones Y2/131, EBM11, Ki-M6 and KP1. Y1/82A is reportedly more specific for monocytes and macrophages than KP1.
Subtype
Primary Antibodies
Target Antigen
CD68
Alternative Names
GP110, LAMP4, Lysosomal-associated membrane protein, Macrosialin, SCARD1, Scavenger receptor class D member 1
Reactive Species
Human
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Granulocytes and Subsets, Myeloid Cells
Species
Human
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
Y1/82A
Gene ID
968
Isotype
IgG2b, kappa
Data Figures
Figure 1. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, followed by anti-hamster (Armenian) IgG, FITC (filled histogram) or an Armenian hamster IgG isotype control antibody followed by anti-hamster (Armenian) IgG, FITC (open histogram).
Figure 2.Data for Alexa Fluor® 488-conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Alexa Fluor® 488 (filled histogram) or an Armenian hamster IgG Alexa Fluor® 488 isotype control antibody (solid line histogram).
Figure 3. Data for APC-conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, APC (filled histogram) or an Armenian hamster IgG APC isotype control antibody (solid line histogram).
Figure 4. Data for Biotin-conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, Biotin followed by streptavidin (SAV) APC (filled histogram) or an Armenian hamster IgG biotin isotype control antibody followed by SAV APC (solid line histogram).
Figure 5. Data for FITC-conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, FITC (filled histogram) or an Armenian hamster IgG FITC isotype control antibody (solid line histogram).
Figure 6. Data for PE-conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PE (filled histogram) or an Armenian hamster IgG PE isotype control antibody (solid line histogram).
Figure 7. Data for PerCP-Cy5.5 Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD3e Antibody, Clone 145-2C11, PerCP-Cy5.5 (filled histogram) or an Armenian hamster IgG isotype control antibody, PerCP-Cy5.5 (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A, APC Catalog #
60105AZ Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A, Biotin Catalog #
60105BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A, FITC Catalog #
60105FI Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A Catalog #
60105, 60105.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A, APC Catalog #
60105AZ Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A, Biotin Catalog #
60105BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A, FITC Catalog #
60105FI Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD68 Antibody, Clone Y1/82A Catalog #
60105, 60105.1 Lot #
All Language
English Resources and Publications
Educational Materials (2)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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