产品号 #60011_C
Mouse (BALB/c) monoclonal IgG1 antibody against human, chimpanzee CD3
概述
The UCHT1 antibody reacts with the ~20 kDa CD3ε subunit of the human T cell receptor (TCR)/CD3 complex, which is expressed on the surface of ~95% of mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severe combined immunodeficiency (SCID).
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Direct Human T Cell Isolation Kit (Catalog #19661), EasySep™ Human CD3 Positive Selection Kit II (Catalog #17851; partial blocking may be observed), EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA) and EasySep™ HLA Whole Blood CD2 Positive Selection Kit (Catalog #18687HLA).
Subtype
Primary Antibodies
Target Antigen
CD3
Alternative Names
CD3e, CD3epsilon, T3
Reactive Species
Chimpanzee, Human
Conjugation
APC, FITC, PE
Host Species
Mouse
Cell Type
T Cells
Species
Human, Non-Human Primate
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
UCHT1
Gene ID
915
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA). Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD2 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibodyfollowed by SAV APC is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibodyfollowed by SAV APC is shown (open histogram).
Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood T Cell Enrichment Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC. Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
Figure 5. Data for PE-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).
Figure 6. Data for Unconjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, followed by goat anti-mouse IgG, FITC (filled histogram). Labeling with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Flow cytometry analysis of PBMCs prior to being processed with EasySep™ Human CD3 Positive Selection Kit (Catalog #18051), which uses Anti-Human CD3 Antibody, Clone UCHT1, to select CD3+ cells. The cells were labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 (Catalog #60016AD) and Anti-Human CD8 Antibody, Clone RPA-T8, PE (Catalog #60022PE).
(C) Flow cytometry analysis of PBMCs processed with EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 (Catalog #60016AD) and Anti-Human CD8 Antibody, Clone RPA-T8 (Catalog #60022PE).
Figure 7. Data for PerCP-Cy55-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD3 Positive Selection Kit (Catalog #18051) and labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 is shown in the bottom panel (solid line histogram).
Figure 8. Data for PerCP-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PE Catalog #
100-0449, 60011PE.1, 60011PE Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, APC Catalog #
60011AZ.1, 60011AZ, 100-0448 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, Biotin Catalog #
60011BT, 60011BT.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, FITC Catalog #
60011FI, 60011FI.1, 100-0447 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1 Catalog #
60011 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP Catalog #
60011PR.1, 60011PR Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5 Catalog #
60011PS.1, 60011PS Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PE Catalog #
100-0449, 60011PE.1, 60011PE Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, APC Catalog #
60011AZ.1, 60011AZ, 100-0448 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, Biotin Catalog #
60011BT, 60011BT.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, FITC Catalog #
60011FI, 60011FI.1, 100-0447 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1 Catalog #
60011 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP Catalog #
60011PR.1, 60011PR Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5 Catalog #
60011PS.1, 60011PS Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Dendritic Cell Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Total Lymphocyte Research
Workflow Stages for Natural Killer Cell Research
Workflow Stages for T Cell Research
Workflow Stages for HLA Analysis
Workflow Stages for T Cell Engineering
Workflow Stages for Chimerism Analysis
Workflow Stages for Innate Lymphoid Cell Research
Resources and Publications
Educational Materials (5)
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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