产品号 #60110_C
Mouse monoclonal IgG1 antibody against human, rhesus, sooty mangabey CD123 (IL-3Rα)
概述
The 6H6 antibody reacts with human CD123 (IL-3 receptor subunit α), an ~70 kDa type I transmembrane glycoprotein belonging to the type I cytokine receptor family (type 5 subfamily) and the immunoglobulin (Ig) superfamily. CD123 constitutes the ligand-binding α chain of the heterodimeric IL-3 receptor. CD123 binds IL-3 with low affinity per se, but when associated with CD131 (the signal-transducing β chain of the receptor), high-affinity binding of IL-3 is observed. CD123 is expressed by hematopoietic progenitor cells, endothelial cells, basophils, eosinophils, mast cells, monocytes, macrophages, dendritic cells, megakaryocytes, a subset of B cells, and by neutrophils if cultured in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-3 binding to CD123 stimulates proliferation, differentiation, and viability of hematopoietic cells. CD123 is highly expressed in malignancies such as acute leukemia. The 6H6 antibody does not inhibit binding of IL-3 to either CD123 or the IL-3 receptor.
Subtype
Primary Antibodies
Target Antigen
CD123 (IL-3Rα)
Alternative Names
hIL-3Ra, IL3R, IL3RAX, IL3RAY, IL3RX, IL3RY, IL-3Ra, IL-3 receptor alpha SP2 isoform, Interleukin-3 Receptor alpha, MGC34174
Reactive Species
Human, Rhesus, Sooty Mangabey
Conjugation
APC, Biotin, FITC, PE, PerCP-Cyanine5.5, Unconjugated
Host Species
Mouse
Cell Type
Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Myeloid Cells
Species
Human, Non-Human Primate
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
6H6
Gene ID
3563
Isotype
IgG1, kappa
Data Figures
Figure 1. Flow Cytometry Analysis of Human PBMCs
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, followed by a rat anti-mouse IgG1 antibody, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 (Catalog #60071) followed by a rat anti-mouse IgG1 antibody, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ).
Figure 2. Data for APC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (Catalog #60071AZ) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 3. Data for Biotin-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, Biotin followed by streptavidin (SAV) APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin (Catalog #60071BT) followed by SAV APC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 4. Data for FITC-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, FITC and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (Catalog #60071FI) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Figure 5. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (Catalog #60071PE) and Anti-Human CD45RO Antibody, Clone UCHL1, APC (Catalog #60097AZ). (C) Flow cytometry analysis (gated on CD45+) of human whole blood nucleated cells processed with the EasySep™ Human Basophil Enrichment Kit (Catalog #19069) and labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE and an anti-IgE antibody, FITC. Labeling of the Start cells prior to cell isolation is shown (D).
Figure 6. Data for PerCP-Cy55-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs, gated on lymphocytes) labeled with Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PerCP-Cy5.5 and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE). (B) Flow cytometry analysis of human PBMCs (gated on lymphocytes) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCPCy5.5 (Catalog #60070PS) and Anti-Human CD45RO Antibody, Clone UCHL1, PE (Catalog #60097PE).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, APC Catalog #
60110AZ, 60110AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, FITC Catalog #
60110FI, 60110FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE Catalog #
60110PE, 60110PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6 Catalog #
60110 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, APC Catalog #
60110AZ, 60110AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, FITC Catalog #
60110FI, 60110FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6, PE Catalog #
60110PE, 60110PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6 Catalog #
60110 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Innate Lymphoid Cell Research
Resources and Publications
Educational Materials (3)
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