产品号 #60039_C
Mouse monoclonal IgG1 antibody against human, mouse CD105 (endoglin)
概述
The 43A3 antibody reacts with CD105 (endoglin), an ~180 kDa cell surface glycoprotein which is a disulfide-bonded homodimer of ~90 kDa type I transmembrane subunits. CD105 is a component of the TGF-β receptor complex and is expressed by vascular endothelial smooth muscle cells, syncytiotrophoblasts of placenta and activated macrophages, and at relatively low levels by stromal fibroblasts. Its expression is also observed in some types of tumors, and levels are up-regulated on the endothelium during angiogenesis. In concert with signaling receptors, CD105 binds to TGF-β1 and TGF-β3 with high affinity, but does not bind TGF-β2. Other ligands reportedly include Activin A, BMP-2, and BMP-7. CD105 has important roles in angiogenesis, cardiovascular development, and vascular remodeling, and the protein serves a regulatory role in cytoskeletal reorganization by modulating the sites of focal adhesion and cellular migration. Certain mutations in CD105 result in the autosomal dominant disorder hereditary hemorrhagic telangiectasia.
This antibody clone has been verified for labeling human mesenchymal cells grown in MesenCult™ Proliferation Kit (Human; Catalog #05411) and MesenCult™-XF Medium (Catalog #05420).
Subtype
Primary Antibodies
Target Antigen
CD105 (Endoglin)
Alternative Names
Endoglin
Reactive Species
Human, Mouse
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesenchymal Stem and Progenitor Cells
Species
Human, Mouse
Application
Flow Cytometry, Immunoprecipitation, Western Blotting
Area of Interest
Endothelial Cell Biology, Stem Cell Biology
Clone
43A3
Gene ID
2022
Isotype
IgG1, kappa
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD105 Antibody, Clone 43A3, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, Alexa Fluor® 488. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, Alexa Fluor® 488 is shown (solid line histogram).
Figure 2. Data for PE-Conjugated
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD105 Antibody, Clone 43A3, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram). (B) Flow cytometry analysis of human PBMCs cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, PE. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, PE is shown (solid line histogram).
Figure 3. Data for Unconjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 hours with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, followed by a rat anti-mouse IgG1 antibody, PE. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, followed by a rat anti-mouse IgG1 antibody, PE is shown (solid line histogram).
Figure 4. Data for APC-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, APC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, APC is shown in the bottom panel (solid line histogram).
Figure 5. Data for Biotin-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a biotinylated mouse IgG1, kappa isotype control antibody followed by SAV APC is shown in the bottom panel (solid line histogram).
Figure 6. Data for FITC-Conjugated
Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) cultured for 24 h with or without lipopolysaccharide (LPS) and labeled with Anti-Human CD105 Antibody, Clone 43A3, FITC. Histograms show labeling of PBMCs cultured in the absence (Unstimulated) or presence (Stimulated) of LPS. Labeling of LPS-stimulated PBMCs with a mouse IgG1, kappa isotype control antibody, FITC is shown in the bottom panel (solid line histogram).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, APC Catalog #
60039AZ, 60039AZ.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, Biotin Catalog #
60039BT Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, FITC Catalog #
60039FI, 60039FI.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, PE Catalog #
60039PE, 60039PE.1 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3 Catalog #
60039 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, APC Catalog #
60039AZ, 60039AZ.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, Biotin Catalog #
60039BT Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, FITC Catalog #
60039FI, 60039FI.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3, PE Catalog #
60039PE, 60039PE.1 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Anti-Human CD105 Antibody, Clone 43A3 Catalog #
60039 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages
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Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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