产品号 #100-0328_C
A cellular protein for detection of apoptotic cells
概述
Annexin V is a member of the annexin family of proteins that bind to membrane phospholipids in the presence of calcium. This dye has high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the plasma membrane, exposing it to the external environment. Annexin V, a characteristic marker for early cell apoptosis, detects the translocation of PS to the external environment.
Annexin V is used along with viability dyes such as 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002). The process of PS translocation occurs prior to the loss of membrane integrity. Therefore, as cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently, viability dyes pass into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and the viability dye.
Conjugation
APC, FITC, PE
Application
Flow Cytometry
Area of Interest
Immunology
Data Figures
Figure 1. Data for Annexin V, APC
Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD (Catalog #75001).
Figure 2. Data for Annexin V, FITC
Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC Annexin V and 7-AAD.
Figure 3. Data for Annexin V, PE
Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with PE Annexin V and 7-AAD.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Annexin V, APC Catalog #
100-0328, 100-0329 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Annexin V, FITC Catalog #
100-0333, 100-0332 Lot #
All Language
English Document Type
Product Information Sheet Product Name
Annexin V, PE Catalog #
100-0330, 100-0331 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Annexin V, APC Catalog #
100-0328, 100-0329 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Annexin V, FITC Catalog #
100-0333, 100-0332 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Annexin V, PE Catalog #
100-0330, 100-0331 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Workflow Stages for Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for Myeloid Cell Research
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