ImmunoCult™-ACF Human B Cell Expansion Supplement
Serum-free and animal component-free culture supplement for expansion of human B cells
ImmunoCult™-ACF Human B Cell Expansion Supplement is a serum-free and animal component-free supplement formulated to promote robust in vitro expansion of human peripheral blood B cells in the absence of serum, feeder cells, or specialized culture plates. The supplement contains recombinant proteins and chemically defined components that support the activation and expansion of human B cells and their maturation to plasma cells.
Available either individually or as part of ImmunoCult™ Human B Cell Expansion Kit. ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF B Cell Base Medium to prepare a complete serum-free medium for culturing and expanding human B cells.
Available either individually or as part of ImmunoCult™ Human B Cell Expansion Kit. ImmunoCult™-ACF Human B Cell Expansion Supplement is a 50X supplement that can be added to a base medium such as ImmunoCult™-XF B Cell Base Medium to prepare a complete serum-free medium for culturing and expanding human B cells.
Data Figures
Figure 1. Expansion and Maturation of Human B Cells with ImmunoCult™ Human B Cell Expansion Kit
B cells isolated from human peripheral blood mononuclear cells (PBMCs) (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit (Catalog #19554) were seeded at 1 x 10^5 cells/well in 24-well tissue culture plates with ImmunoCult™-ACF Human B Cell Expansion Supplement and ImmunoCult™-XF B Cell Base Medium included in the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 3 - 4 days. (A) Fold expansion of viable cells is shown for n = 12 donors, with bars representing the mean and 95% confidence level (range 38- to 1190-fold at day 14 ± 1 day). (B) Expression of CD138 and CD20 was analyzed by flow cytometry at each timepoint (data represent % positive viable cells; mean ± 1 SD). The observed changes indicate maturation of B cells to plasma cells/blasts.
Figure 2. Light Microscopy Image of Cultured Human B Cells
B cells isolated from human PBMCs (leukopak) using EasySep™ Human Pan-B Cell Enrichment Kit were seeded at 1 x 10^5 cells/well in a 24-well tissue culture plate and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged on day 4 after seeding and imaged at 40X magnification on day 6.
Figure 3. Expansion and Viability of Human Memory B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit
Memory B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Figure 4. Expansion and Viability of Human Naïve B Cells Cultured with ImmunoCult™ Human B Cell Expansion Kit
Naïve B cells were isolated from human PBMCs (leukopak) using EasySep™ Human Memory B Cell Isolation Kit and were seeded at 0.5 x 10^5 cells/well in 48-well tissue culture plates and cultured with the ImmunoCult™ Human B Cell Expansion Kit. The cells were passaged every 2 - 4 days and the fold expansion of viable cells (A) and cell viability (B) were calculated at each timepoint. Data represent the mean ± 1 SD of triplicate cultures for the same donor.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Product Information Sheet 2
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™-ACF Human B Cell Expansion Supplement
Catalog #
10974
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Resources and Publications
Educational Materials (7)
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