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StemSpan™-ACF Erythroid Expansion Medium

Animal component-free medium for culture and expansion of human erythroid cells
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产品号 #09860_C

Animal component-free medium for culture and expansion of human erythroid cells

产品优势


  • Animal component-free conditions specifically designed for expansion and differentiation of erythroid progenitor cells

  • Optimized for use with StemSpan™ Erythroid Expansion Supplement

  • Produces thousands of CD71+GlyA+ erythroid cells per input CD34+ cell

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StemSpan™-ACF Erythroid Expansion Medium
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

StemSpan™-ACF Erythroid Expansion Medium (ACF-E) is an animal component-free (ACF) medium for the in vitro culture and expansion of human hematopoietic cells. When supplemented with StemSpan™ Erythroid Expansion Supplement (100X; Catalog #02692), this medium supports the expansion and erythroid differentiation of human hematopoietic progenitor cells into CD71+ and Glycophorin A+ erythroblasts in ACF culture conditions. This medium only contains recombinant proteins and synthetic components; it does not contain serum or other human- or animal-derived components.

Contains
<div> This product contains only recombinant proteins and synthetic components
 
Subtype
Specialized Media
 
Cell Type
Erythroid Cells, Hematopoietic Stem and Progenitor Cells
 
Species
Human
 
Application
Cell Culture, Differentiation, Expansion
 
Brand
StemSpan
 
Area of Interest
Stem Cell Biology, Transplantation Research
 
Formulation Category
Animal Component-Free, Serum-Free, Xeno-Free
 

Data Figures

Figure 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.

Table 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA.

*CI: Confidence Interval.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
StemSpan™-ACF Erythroid Expansion Medium
Catalog #
09860
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
StemSpan™-ACF Erythroid Expansion Medium
Catalog #
09860
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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