STEMdiff™ Lung Progenitor Kit is a serum-free culture medium system for efficient and reproducible generation of lung progenitor cells from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) through three stages of differentiation: 1) definitive endoderm, 2) anterior foregut endoderm, and 3) lung progenitor cells. Differentiated cells will express NKX2.1, a key marker of lung progenitor cells. The resulting cells can be further matured towards proximal or distal airway cells using published protocols for the study of lung diseases and lung development.
Area of Interest Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Respiratory Research, Stem Cell Biology
Formulation Category Serum-Free
Data Figures
Figure 1. Generation of Lung Progenitor Cells from hESCs/hiPSCs with the STEMdiff™ Lung Progenitor Kit
Human embryonic (hESCs) or induced (hiPSCs) pluripotent stem cell cultures progress through a simple three-stage process to generate lung progenitor cells. hESCs or hiPSCs clumps are first seeded in mTeSR™1 or mTeSR™ Plus. On Day 1, differentiation is initiated with Medium DE-1 (STEMdiff™ Endoderm Basal Medium containing Supplement MR and Supplement CJ). Subsequently, on Days 2 and 3, the medium is changed to Medium DE-2 (STEMdiff™ Endoderm Basal Medium containing Supplement CJ) for definitive endoderm patterning. On Day 4, to initiate anterior foregut endoderm patterning, the endoderm monolayer is passaged in Medium LP-1 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 1) and Y-27632 (Dihydrochloride). Finally, at Day 7, the cells are differentiated into the lung progenitor stage with Medium LP-2 (STEMdiff™ Lung Basal Medium, Lung Supplement (10X), and Supplement 2).
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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