产品号 #08007_C
扩增人多能干细胞衍生的内皮细胞
总览
STEMdiff™ 内皮细胞扩增培养基试剂盒（目录号 #08007）包含无异种成分的内皮细胞扩增基础培养基和补充剂，必须与无动物成分细胞贴壁基质配合使用。STEMdiff™ 内皮细胞扩增培养基针对 hPSC 衍生内皮细胞的扩增和内皮细胞标志物表达的维持进行了优化。传代培养时，需要使用无动物成分细胞解离试剂盒（目录号 #05426）。
注：
制备整的 STEMdiff™ 内皮扩增培养基时需使用肝素溶液（目录号 #07980）。
细胞附着底物中的 rhCollagen 成分由 CollPlant 公司生产。
本产品仅供研究使用。如有任何临床或商业用途，请联系 STEMCELL。
亚型
专用培养基
细胞类型
内皮细胞
种属
人
应用
细胞培养，扩增
品牌
STEMdiff
研究领域
血管生成细胞研究，疾病建模，内皮细胞研究
制剂类别
无血清
Data Figures
Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit
In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).
Figure 2. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium
STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
STEMdiff™ Endothelial Expansion Medium Kit Catalog #
08007 Lot #
All Language
English Document Type
Safety Data Sheet 1 Product Name
STEMdiff™ Endothelial Expansion Medium Kit Catalog #
08007 Lot #
All Language
English Document Type
Safety Data Sheet 2 Product Name
STEMdiff™ Endothelial Expansion Medium Kit Catalog #
08007 Lot #
All Language
English Document Type
Safety Data Sheet 3 Product Name
STEMdiff™ Endothelial Expansion Medium Kit Catalog #
08007 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Resources and Publications
Educational Materials (2)
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更多信息
| Species | Human |
|---|---|
| Formulation Category | Serum-Free |
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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