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STEMdiff™ 内皮分化试剂盒

高效将人多能干细胞分化为内皮细胞
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产品号 #08005_C

高效将人多能干细胞分化为内皮细胞

产品优势

  • 高效生成 hPSC 衍生的内皮细胞。
  • 无需细胞富集或分选步骤。
  • 与含有 FBS 的培养基相比，hPSC 衍生的内皮细胞扩增效果更优异。

产品组分包括

  • STEMdiff™ 内皮诱导培养基试剂盒（目录号 #08005）
    • STEMdiff™ 内皮诱导培养基，100 mL
    • STEMdiff™ 内皮扩增基础培养基，120 mL
    • STEMdiff™ 内皮扩张 5X 补充剂，30 mL
    • 无动物成分细胞贴壁底物，1 mL
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

STEMdiff™ 内皮细胞分化试剂盒（货号 #08005）包含贴壁底物、无动物成分 (ACF) 内皮细胞诱导培养基和内皮细胞扩增培养基。该试剂盒经过优化，可用于将在康宁Matrigel® 基质上以 mTeSR™1（目录号 #85850）、mTeSR™ Plus（目录号 #05825）或 TeSR™-E8™（目录号 #05990）中维持的人多能干细胞（hPSCs）分化为内皮样细胞。该试剂盒设计用于在使用 STEMdiff™ 中胚层诱导培养基（目录号 #05220，可单独购买）进行早期中胚层诱导后立即进行内皮诱导培养。细胞贴壁底物中的 rhCollagen 成分由 CollPlant 公司生产。

本产品仅供研究使用。如有任何临床或商业应用需求，请联系 STEMCELL。

亚型
专用培养基
 
细胞类型
内皮细胞
 
种属
人
 
应用
分化
 
品牌
STEMdiff
 
研究领域
血管生成细胞研究，疾病建模，内皮细胞研究
 
制剂类别
无血清
 

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Data Figures

Figure 1. Schematic Workflow of Endothelial Induction Using the STEMdiff™ Endothelial Kit

In Phase 1, human embryonic stem (ES) or induced pluripotent stem (iPS) cells are cultured in a TeSR™ maintenance medium (mTeSR™ Plus, mTeSR™1, or TeSR™-E8™). On Day 1 (Phase 2) of the protocol, cells are ready for induction into early mesoderm progenitor cells by replacing TeSR™ medium with STEMdiff™ Mesodermal Induction Medium (MIM). By Day 3 (Phase 3), STEMdiff™ Mesoderm Induction Medium is replaced with STEMdiff™ Endothelial Induction Medium to derive endothelial cells. On Day 7, cells are passaged 5 - 6 times onto cultureware pre-coated with Animal Component-Free Cell Attachment Substrate in STEMdiff™ Endothelial Expansion Medium (Phase 4).

Figure 2. A Representative Flow Cytometric Analysis of Endothelial Marker Expression in hPSC-Derived Endothelial Cells

Human pluripotent stem cell (hPSC; H9 cell line)-derived endothelial cells were obtained at Day 7 using STEMdiff™ Endothelial Induction Medium. Greater than 85% of the cells were CD34+ and had high levels of CD31 and CD144 expression. With subsequent passages, the proportion of cells expressing endothelial markers (CD34+, CD31, and CD144) increased up to passage 5.

Figure 3. STEMdiff™ Endothelial Differentiation Kit Generates Functional hPSC-Derived Endothelial Cells

Endothelial cells generated from hPSCs (F016 cell line) using the STEMdiff™ Endothelial Differentiation Kit take up acetylated LDL when plated at 10,000 cells/cm2. Cells are able to form tubular networks in vitro in a tube formation assay when plated at 20,000 cells/well in a 96 well-plate for 24 hrs.

Figure 4. Endothelial Cells Expand Faster in STEMdiff™ Endothelial Expansion Medium Compared to Serum-Containing Medium

STEMdiff™ Endothelial Expansion Medium (A) sustains expansion rate in later passages and leads to (B) superior expansion of hPSC (C1 cell line)-derived endothelial cells when compared to serum-containing medium.

Figure 5. hPSC-Derived Endothelial Cells Generated Using the STEMdiff™ Endothelial Differentiation Kit Express High Levels of ACE2

(A) hPSC (C1 cell line)-derived endothelial cells were generated using the STEMdiff™ Endothelial Differentiation Kit and expanded in STEMdiff™ Endothelial Expansion Medium for 6 passages at 10,000 cells/cm2. (B) The cells were then analyzed for expression of angiotensin-converting enzyme 2 (ACE2). 85% of cells expressed high levels of ACE2.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Endothelial Differentiation Kit
Catalog #
08005
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Endothelial Differentiation Kit
Catalog #
08005
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Endothelial Differentiation Kit
Catalog #
08005
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Endothelial Differentiation Kit
Catalog #
08005
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Endothelial Differentiation Kit
Catalog #
08005
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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