STEMdiff™ Blood Vessel Organoid Kit

Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids

产品号 #100-0651_C

Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids

产品优势


  • Generates physiologically relevant 3D blood vessel organoids ideal for disease modeling and drug discovery

  • Provides a standardized protocol with optimized reagents for efficient generation of hPSC-derived organoids

  • Supports high-throughput scalability in a 96-well format for drug discovery or drug testing

产品组分包括

  • STEMdiff™ Blood Vessel Organoid Aggregation Basal Medium, 60 mL
  • STEMdiff™ Blood Vessel Organoid Aggregation Supplement, 15 mL
  • STEMdiff™ Blood Vessel Organoid Induction Basal Medium, 100 mL
  • STEMdiff™ Blood Vessel Organoid Induction Supplement, 1 mL
  • STEMdiff™ Blood Vessel Organoid Mesodermal Induction Supplement, 1 mL
  • STEMdiff™ Blood Vessel Organoid Vascular Induction Supplement, 1 mL
  • STEMdiff™ Blood Vessel Organoid Maturation Medium, 100 mL (Catalog #100-0658)

概述

Generate physiologically relevant blood vessel organoids—easily and with high yield—for your tissue repair and regeneration studies or to model vascular disease in vitro by using STEMdiff™ Blood Vessel Organoid Kit.

Use STEMdiff™ Blood Vessel Organoid Kit for efficient and reproducible generation of blood vessel organoids from human pluripotent stem cells (hPSCs), with the option to scale up for high-throughput screening in a 96-well format. Compared to homemade organoid culture media formulations, organoids generated with this easy-to-use kit develop extensive and physiologically relevant vascular networks and strong endothelial cell-pericyte interactions. Blood vessel organoids generated using this kit have CD31+/CD34+/CD144+/KDR+ endothelial cells and PDGFR-β+/CD146+/SMA+/NG-2+ pericytes.The simple protocol also eliminates the need for serum screening and labor-intensive protocol optimization, so you can spend more time on what matters—your experiments.

Use STEMdiff™ Blood Vessel Organoid Kit with mTeSR™ Plus or mTeSR™1 culture media for an easy transition from hPSCs to organoids. For your convenience, STEMdiff™ Blood Vessel Organoid Maturation Medium, required for the maturation of hPSC-derived blood vessel organoids, is available either as a part of the kit or for individual sale. To differentiate hPSCs to endothelial cells, use our STEMdiff™ Endothelial Differentiation Kit.

Subtype
Specialized Media, Supplements
 
Cell Type
Endothelial Cells, Endothelial Cells, PSC-Derived
 
Species
Human
 
Application
Functional Assay, Organoid Culture, Toxicity Assay
 
Brand
STEMdiff
 
Area of Interest
Angiogenic Cell Research, Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology
 

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Technical Manual
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Safety Data Sheet 1
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Safety Data Sheet 2
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Safety Data Sheet 5
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Safety Data Sheet 6
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Safety Data Sheet 7
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Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Legal Statement: This product was developed under a license to intellectual property owned by the Institute of Molecular Biotechnology (IMBA) of the Austrian Academy of Sciences. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services or, in collaboration with, a for-profit entity, for purposes other than research applications (i.e., drug development activities). Purchasers wishing to use the product for commercial purposes should contact IMBA at technologvtransfer@imba.oeaw.ac.at. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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