Serum-free culture medium kit for differentiation of human PSCs to atrial cardiomyocytes
产品优势
Simple monolayer protocol, compatible with STEMdiff™ cardiomyocyte workflow
Establishment and efficient generation of up to 50 million atrial cardiomyocytes per kit
Cardiomyocytes generated with this serum-free kit express high levels of atrial-specific genes and demonstrate dominant atrial-like phenotypes including faster beat rate and decreased field potential duration when compared to those generated using the STEMdiff™ Cardiomyocyte Differentiation Kit
Maximum reproducibility with minimal variability across multiple hPSC lines
产品组分包括
STEMdiff™ Cardiomyocyte Differentiation Basal Medium, 380 mL
STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement A (10X), 10 mL
STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement B (10X), 10 mL
STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement C (10X), 20 mL
STEMdiff™ Cardiomyocyte Maintenance Basal Medium, 490 mL
STEMdiff™ Atrial Cardiomyocyte Maintenance Supplement (50X), 10 mL
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit is optimized for the differentiation and maintenance of human embryonic stem and induced pluripotent stem cells into atrial cardiomyocytes (cardiac troponin T-positive; cTnT+). This serum-free kit can be used to generate atrial cardiomyocytes derived from a clump culture of human pluripotent stem cells (hPSCs) maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990). With greater than 80% yield purity, an average of 1 x 10^6 cTnT+ cells can be harvested from a single well of a 12-well plate.
STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.
Subtype Specialized Media
Cell Type Cardiomyocytes, PSC-Derived
Species Human
Application Cell Culture, Differentiation
Brand STEMdiff
Area of Interest Cell Line Development, Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
Formulation Category Serum-Free
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
