产品号 #72242_C
表观遗传修饰剂；抑制组蛋白去乙酰化酶
总览
丁酸钠是丁酸的钠盐。丁酸是一种短链脂肪酸，可抑制组蛋白去乙酰化酶 (HDAC)，导致组蛋白过度乙酰化。这会导致染色质结构和基因表达发生变化，从而产生多种生物学效应。(Boffa et al.; Kruh)
重编程
·仅使用单一因子 OCT4 即可促进人体细胞重编程为诱导多能干细胞 (iPS)（Zhu et al.）。
维持和自我更新
·在没有外源添加生长因子的情况下，支持小鼠和人胚胎干细胞 (ES) 的自我更新（Ware et al）。
分化
·促进小鼠和人 ES 细胞分化为肝细胞 （Hay et al.; Zhou et al.)。
·促进人 ES 细胞分化为定形内胚层和胰岛样细胞（Jiang et al.）。
·增强成骨作用并抑制人间充质细胞的脂肪形成分化（Chen et al.; Lee et al.)。
细胞类型
内胚层，PSC衍生，肝细胞，间充质干/祖细胞，成骨细胞，多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化，扩增，培养，重编程
研究领域
上皮细胞研究，干细胞生物学
CAS 编号
156-54-7
化学式
C₄H₇O₂ · Na
纯度
≥ 95 %
通路
表观遗传学
靶点
HDAC
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (9)
Differentiation of mouse embryonic stem cells into hepatocytes induced by a combination of cytokines and sodium butyrate. Journal of cellular biochemistry 2010 FEB
Abstract
There is increasing evidence to suggest that embryonic stem cells (ESCs) are capable of differentiating into hepatocytes in vitro. In this study, we used a combination of cytokines and sodium butyrate in a novel three-step procedure to efficiently direct the differentiation of mouse ESCs into hepatocytes. Mouse ESCs were first differentiated into definitive endoderm cells by 3 days of treatment with Activin A. The definitive endoderm cells were then differentiated into hepatocytes by the addition of acidic fibroblast growth factor (aFGF) and sodium butyrate to the culture medium for 5 days. After 10 days of further in vitro maturation, the morphological and phenotypic markers of hepatocytes were characterized using immunohistochemistry, immunoblotting, and reverse transcription-polymerase chain reaction (RT-PCR). Furthermore, the cells were tested for functions associated with mature hepatocytes, including glycogen storage and indocyanine green uptake and release, and the ratio of hepatic differentiation was determined by counting the percentage of albumin-positive cells. In the presence of medium containing cytokines and sodium butyrate, numerous epithelial cells resembling hepatocytes were observed, and approximately 74% of the cells expressed the hepatic marker, albumin, after 18 days in culture. RT-PCR analysis and immunohistochemistry showed that these cells expressed adult liver cell markers, and had the abilities of glycogen storage and indocyanine green uptake and release. We have developed an efficient method for directing the differentiation of mouse ESCs into cells that exhibit the characteristics of mature hepatocytes. This technique will be useful for research into the molecular mechanisms underlying liver development, and could provide a source of hepatocytes for transplantation therapy and drug screening.
Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell stem cell 2010 DEC
Abstract
Histone deacetylase inhibitors decrease proliferation potential and multilineage differentiation capability of human mesenchymal stem cells. Cell proliferation 2009 DEC
Abstract
OBJECTIVES Histone deacetylase (HDAC) is an important therapeutic target in cancer. Two of the main anticancer mechanisms of HDAC inhibitors are induction of terminal differentiation and inhibition of cell proliferation. To investigate the role of HDAC in maintenance of self-renewal and cell proliferation, we treated mesenchymal stem cells (MSCs) that originated from adipose tissue or umbilical cord blood with valproic acid (VPA) and sodium butyrate (NaBu). MATERIALS AND METHODS Human MSCs were isolated from mammary fat tissue and cord blood. We performed MTT assay and flow cytometry-based cell cycle analysis to assess self-renewal of MSCs. In vitro differentiation assays into osteogenic, adipogenic, neurogenic and chondrogenic lineages were conducted to investigate MSC multipotency. Immunocytochemistry, Western blot and reverse transcription-polymerase chain reaction were used to interrogate molecular pathways. RESULTS VPA and NaBu flattened the morphology of MSCs and inhibited their growth. VPA and NaBu activated the transcription of p21(CIP1/WAF1) by increasing the acetylation of histone H3 and H4 and eventually blocked the cell cycle at G2/M phase. The expression level of p16(INK4A), a cdk inhibitor that is closely related to cellular senescence, was not changed by HDAC inhibitor treatment. We performed controlled differentiation into bone, fat, cartilage and nervous tissue to elucidate the role of HDAC in the pluripotency of MSC to differentiate into functional tissues. VPA and NaBu decreased the efficiency of adipogenic, chondrogenic, and neurogenic differentiation as visualized by specific staining and reverse transcription-polymerase chain reaction. In contrast, osteogenic differentiation was elevated by HDAC inhibitor treatment. CONCLUSION HDAC activity is essential for maintaining the self-renewal and pluripotency of MSCs.
更多信息
| Species | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 156-54-7 |
| Chemical Formula | C₄H₇O₂ · Na |
| Purity | ≥ 95% |
| Target | HDAC |
| Pathway | Epigenetic |
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