产品号 #100-0994_C
一种细胞渗透性钙染料，在Ca2+浓度浓度升高时会发出荧光
使用Rhod-2 AM（溴化物）荧光染料检测线粒体钙离子水平的变化，并使用荧光显微镜进行检测验证。
特点:
•激发波长：553nm
•发射波长：577nm
•解离常数(Kd) = 570 nM
• 已验证可用于荧光显微镜
Rhod-2 AM是一种细胞渗透性乙酰氧基甲基（AM）酯染料，在Ca2+存在下发出荧光。AM基团促进了Rhod-2 AM的细胞摄取，该基团被胞质酯酶去除，从而在细胞内积聚（Smith等，Sci Signal，2018）。Rhod-2 AM选择性地在线粒体中积累。当 Ca²⁺ 浓度升高时，会显著增强 Rhod-2 AM 的荧光信号。该染料较长的发射波长使其在高自发荧光背景的细胞实验中具有较高的应用价值。
细胞内钙离子浓度的升高是细胞凋亡通路激活的重要指标（Mukherjee 等人，J Biol Chem，2002）。使用Rhod-2 AM（溴化物）染料通过流式细胞术、荧光显微镜和微孔板阅读器检测来检测线粒体钙内流。
特点:
•激发波长：553nm
•发射波长：577nm
•解离常数(Kd) = 570 nM
• 已验证可用于荧光显微镜
Rhod-2 AM是一种细胞渗透性乙酰氧基甲基（AM）酯染料，在Ca2+存在下发出荧光。AM基团促进了Rhod-2 AM的细胞摄取，该基团被胞质酯酶去除，从而在细胞内积聚（Smith等，Sci Signal，2018）。Rhod-2 AM选择性地在线粒体中积累。当 Ca²⁺ 浓度升高时，会显著增强 Rhod-2 AM 的荧光信号。该染料较长的发射波长使其在高自发荧光背景的细胞实验中具有较高的应用价值。
细胞内钙离子浓度的升高是细胞凋亡通路激活的重要指标（Mukherjee 等人，J Biol Chem，2002）。使用Rhod-2 AM（溴化物）染料通过流式细胞术、荧光显微镜和微孔板阅读器检测来检测线粒体钙内流。
Protocols and Documentation
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| Molecular Weight | 1124.0 g/mol |
|---|---|
| Cas Number | 145037-81-6 |
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