产品号 #07516_C
适用于探针法检测与微阵列分析
总览
qPCR 预混液是一种2X浓缩溶液，针对基于TaqMan®探针的实时定量聚合酶链反应（qPCR）。它需要与基因特异性引物（扩增目标cDNA）和荧光标记探针（利用Taq DNA聚合酶的5 '核酸酶活性产生荧光信号）结合使用。荧光信号的积累速率用于定量cDNA，从而确定原始样本中mRNA的含量。qPCR预混液包括热启动DNA聚合酶，dNTPs, MgCl2，增强剂和稳定剂。qPCR预混液试剂盒还包括ROX参考染料作为单独的组分，使其兼容依赖或非依赖参考染料的qPCR系统。• 高效、灵敏且可重复 • 在标准或快速循环条件下均具有最佳性能 • 非常适合高通量应用和过夜实验 • 兼容各种 qPCR 仪器
细胞类型
脂肪细胞，软骨细胞，内胚层，PSC衍生，肝细胞，肠道细胞，间充质干/祖细胞，成骨细胞，胰腺细胞，多能干细胞
种属
人
应用
基因组编辑
研究领域
干细胞生物学
Data Figures
Figure 1. PCR Amplification Efficiency Remains Consistently High Under Standard or Fast Cycling Conditions
qPCR was performed with qPCR array plates, template, qPCR Master Mix and reference dye using a real-time PCR instrument. (A) The calculated PCR efficiency of 13 assays (n = 26) is shown run under fast cycling conditions using either diluted cDNA (50–0.016 ng) or 101 –107 copies of template. All assays exhibited 90–110% PCR efficiency with R2 >0.99. (B) At each concentration of cDNA (50 – 0.016 ng; 3 of 6 dilutions shown), the difference in Cq values determined using standard or fast cycling conditions was <1. Standard cycling: 3 min. 95°C; 49 x (15 sec. 95°C; 1 min. 60°). Fast cycling: 3 min. 95°C; 49 x (5 sec. 95°C; 30 sec. 60°).
Figure 2. Reproducible Amplification with qPCR Master Mix After Extended Pre-Heating
qPCR Master Mix was either heated at 55°C for 4 or 8 hours or not heated before use in qPCR assays with reference dye and varying amounts of cDNA (50 - 0.08 ng). An overlay of the amplification plots shows no effect on the amplification curves for unheated or heated qPCR Master Mix.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
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Document Type
Product Information Sheet Product Name
qPCR Master Mix Kit Catalog #
07517, 07516 Lot #
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English Document Type
Safety Data Sheet 1 Product Name
qPCR Master Mix Kit Catalog #
07517, 07516 Lot #
All Language
English Document Type
Safety Data Sheet 2 Product Name
qPCR Master Mix Kit Catalog #
07517, 07516 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Human Pluripotent Stem Cell Research
Workflow Stages for hPSC-Derived Endoderm Cell Research
Workflow Stages for hPSC-Derived Neural Cell Research
Workflow Stages for hPSC-Derived Mesoderm Cell Research
Workflow Stages for Mesenchymal Stem and Progenitor Cell Research
Workflow Stages for Extracellular Vesicle Research
Resources and Publications
Educational Materials (2)
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| Species | Human |
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