切换导航
  • 比较产品
我的购物车

StemSpan™ T细胞生成试剂盒

将人CD34+造血祖细胞扩增并分化为T细胞的全套试剂盒
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #09940_C

将人CD34+造血祖细胞扩增并分化为T细胞的全套试剂盒

产品组分包括

  • StemSpan™ SFEM II培养基，2 x 100 mL
  • StemSpan™淋系祖细胞扩增添加物（10X），5 mL
  • StemSpan™淋系祖细胞分化包板材料（100X），2 x 0.25 mL
  • StemSpan™T细胞祖细胞成熟添加物（10X），12.5 mL
main product photo
总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

采用两阶段的分化方法，可从起始的人脐带血（CB）CD34+细胞稳定扩增出数千个CD4+CD8+双阳性（DP）T细胞。

第一阶段中，CB CD34+细胞在含扩增添加物的培养基中培养14天，促进其增殖并分化为CD7+CD5+ T祖细胞。第二阶段中，将前14天生成的T祖细胞在含成熟添加物的培养基中继续培养4周，促使其成熟为DP细胞。该培养基试剂盒无血清且无需饲养层，消除了血清和基质细胞系引入的不稳定因素。

所有试剂盒组分可单独购买以方便您的使用。StemSpan™淋系祖细胞扩增添加物（10X）包含重组人细胞因子组合和其他添加剂，专用于选择性促进从人脐带血（CB）或骨髓（BM）样本中分离的CD34+细胞扩增及分化为CD7+CD5+ T祖细胞（pro-T）。此外，StemSpan™ T细胞祖细胞成熟添加物（10X）能进一步促使T祖细胞成熟为DP细胞，以及在进一步的刺激条件下支持DP细胞成熟为CD8单阳性（SP）T细胞。StemSpan™淋系祖细胞扩增添加物（10X）和StemSpan™ T细胞祖细胞成熟添加物（10X）需与StemSpan™ SFEM II培养基以及用StemSpan™淋系分化包板材料（100X）包被的培养板搭配使用。

可选的将DP细胞进一步成熟为CD8 SP T细胞的延长实验方法需要使用由StemSpan™ T细胞生成试剂盒所含试剂配制的完全T细胞祖细胞成熟培养基，并需要搭配使用ImmunoCult™ CD3/CD28/CD2 T细胞激活剂和IL-15。

Subtype
Specialized Media, Supplements
 
Cell Type
Hematopoietic Stem and Progenitor Cells, T Cells
 
Species
Human
 
Application
Cell Culture, Differentiation, Expansion
 
Brand
StemSpan
 
Area of Interest
Cancer, Immunology, Stem Cell Biology, Cell Therapy Development
 
Formulation Category
Serum-Free
 

查看更多 显示更少

Data Figures

Figure 1. Frequency and Yield of CD7+CD5+ Pro-T Cells After 14 Days of Culture

CB-derived CD34+ cells (freshly isolated or frozen) were cultured for 14 days in StemSpan™ SFEM II containing Lymphoid Progenitor Expansion Supplement (Catalog #09915) on plates coated with Lymphoid Differentiation Coating Material (Catalog #09925). Cells were harvested and analyzed for CD7 and CD5 expression by (A) flow cytometry. The (B) average frequency of viable CD7+CD5+ pro-T cells on day 14 was 70%, with ~200 CD7+CD5+ cells produced per input CD34+ cell. Shown are means with 95% confidence intervals (n = 33).

Figure 2. Frequency and Yield of CD4 ISP and CD4+CD8+ DP Cells After 42 Days of Culture

CB-derived CD34+ cells (freshly isolated or frozen) were cultured with the StemSpan™ T Cell Generation Kit (Catalog #09940) for 42 days and (A) analysed by flow cytometry for the expression of CD4, CD8, CD3 and TCRαβ. The (B) frequency and (C) yield of CD4 ISP, double-positive (CD4+CD8+) and CD3+TCRαβ+-expressing double-positive cells (CD4+CD8+CD3+TCRαβ+) are shown. On average, 38% of the total viable population were DP (CD4+CD8+), of which 35% co-expressed CD3 and TCRαβ. The yields of total DP cells and CD3+TCRαβ+ DP cells per input CD34+ cell were ~23,000 and ~9,000, respectively. Shown are means with 95% confidence intervals (n = 31).

Figure 3. Frequency and Yield of CD8 SP T Cells After 49 Days of Culture

DP cells were further matured into CD8 SP T cells by culturing for an additional 7 days in StemSpan™ SFEM II with T Cell Progenitor Maturation Supplement (Catalog #09930), IL-15 (Catalog #78031) and ImmunoCult™ CD3/CD28/CD2 T Cell Activator (Catalog #10970) on coated plates. On day 49, cells were (A) analyzed by flow cytometry for the expression of CD3, TCRαβ, CD4 and CD8. The (B) frequency and yield of CD3+TCRαβ+-expressing cells and their subsets are shown. On average, 54% of the CD3+TCRαβ+ cells were DP (CD4+CD8+) and 38% were CD8 SP (CD4-CD8+). The average yield of CD8 SP T cells per input CD34+ cell was ~6,000. CD3+TCRαβ+ CD4 SP (CD4+CD8-) T cells were detected at very low frequencies (data not shown). Shown are means with 95% confidence intervals (n = 12).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
StemSpan™ T Cell Generation Kit
Catalog #
09940
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
StemSpan™ T Cell Generation Kit
Catalog #
09940
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
StemSpan™ T Cell Generation Kit
Catalog #
09940
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
StemSpan™ T Cell Generation Kit
Catalog #
09940
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
StemSpan™ T Cell Generation Kit
Catalog #
09940
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
StemSpan™ T Cell Generation Kit
Catalog #
09940
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。