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StemSpan™淋系祖细胞分化包板材料（100X）

用于淋系祖细胞扩增与分化的包板材料
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产品号 #09925_C

用于淋系祖细胞扩增与分化的包板材料

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概述

使用StemSpan™淋系祖细胞分化包板材料（100X）包被培养板，以将人CD34+造血祖细胞扩增和分化为T细胞。

该产品是StemSpan™ T细胞生成试剂盒或STEMdiff™ T细胞分化试剂盒的组分之一，也可单独购买以方便您的使用。在本实验流程中，首先使用StemSpan™淋系祖细胞扩增添加物（10X）将CD34+细胞分化为CD7+CD5+淋系祖细胞，随后进一步定向分化为CD4+CD8+双阳性（DP） T细胞。

StemSpan™淋系祖细胞分化包板材料（100X）需与StemSpan™ T细胞祖细胞成熟添加物（10X）、StemSpan™ SFEM II培养基以及StemSpan™淋系祖细胞扩增添加物（10X）搭配使用。

关于使用StemSpan™进行CD34+细胞扩增与分化的详细方法，请参阅技术手册与教育资源。

Subtype
Supplements
 
Cell Type
Hematopoietic Stem and Progenitor Cells
 
Species
Human
 
Application
Cell Culture
 
Brand
StemSpan
 
Area of Interest
Cancer, Immunology, Stem Cell Biology, Cell Therapy Development
 
Formulation Category
Serum-Free
 

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Data Figures

Figure 1. Frequency and Yield of CD7+CD5+ Pro-T Cells After 14 Days of Culture

CB-derived CD34+ cells (freshly isolated or frozen) were cultured for 14 days in StemSpan™ SFEM II containing Lymphoid Progenitor Expansion Supplement (Catalog #09915) on plates coated with Lymphoid Differentiation Coating Material (Catalog #09925). Cells were harvested and analyzed for CD7 and CD5 expression by (A) flow cytometry. The (B) average frequency of viable CD7+CD5+ pro-T cells on day 14 was 70%, with ~200 CD7+CD5+ cells produced per input CD34+ cell. Shown are means with 95% confidence intervals (n = 33).

Figure 2. Frequency and Yield of CD4 ISP and CD4+CD8+ DP Cells After 42 Days of Culture

CB-derived CD34+ cells (freshly isolated or frozen) were cultured with the StemSpan™ T Cell Generation Kit (Catalog #09940) for 42 days and (A) analysed by flow cytometry for the expression of CD4, CD8, CD3 and TCRαβ. The (B) frequency and (C) yield of CD4 ISP, double-positive (CD4+CD8+) and CD3+TCRαβ+-expressing double-positive cells (CD4+CD8+CD3+TCRαβ+) are shown. On average, 38% of the total viable population were DP (CD4+CD8+), of which 35% co-expressed CD3 and TCRαβ. The yields of total DP cells and CD3+TCRαβ+ DP cells per input CD34+ cell were ~23,000 and ~9,000, respectively. Shown are means with 95% confidence intervals (n = 31).

Figure 3. Frequency and Yield of CD8 SP T Cells After 49 Days of Culture

DP cells were further matured into CD8 SP T cells by culturing for an additional 7 days in StemSpan™ SFEM II with T Cell Progenitor Maturation Supplement (Catalog #09930), IL-15 (Catalog #78031) and ImmunoCult™ CD3/CD28/CD2 T Cell Activator (Catalog #10970) on coated plates. On day 49, cells were (A) analyzed by flow cytometry for the expression of CD3, TCRαβ, CD4 and CD8. The (B) frequency and yield of CD3+TCRαβ+-expressing cells and their subsets are shown. On average, 54% of the CD3+TCRαβ+ cells were DP (CD4+CD8+) and 38% were CD8 SP (CD4-CD8+). The average yield of CD8 SP T cells per input CD34+ cell was ~6,000. CD3+TCRαβ+ CD4 SP (CD4+CD8-) T cells were detected at very low frequencies (data not shown). Shown are means with 95% confidence intervals (n = 12).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
StemSpan™ Lymphoid Differentiation Coating Material (100X)
Catalog #
09925
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
StemSpan™ Lymphoid Differentiation Coating Material (100X)
Catalog #
09925
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
StemSpan™ Lymphoid Differentiation Coating Material (100X)
Catalog #
09925
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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