RSeT™ 无饲养层培养基

类原始态人多能干细胞的无血清培养基

产品号 #(选择产品)

产品号 #05975_C

类原始态人多能干细胞的无血清培养基

产品优势

无饲养层培养体系,减少了饲养层制备带来的固有变异性、成本和操作负担

无血清配方,采用预筛选的高质量组分,确保结果具有良好重复性

高效促进细胞回转至类原始态,细胞呈现稳定的圆顶状形态,原始态基因表达谱,且无需外源基因即可保持低水平自发分化

在不添加bFGF的情况下,维持类原始态的多能性

产品组分包括

  • RSeT™ 无饲养层基础培养基,450 mL
  • RSeT™ 无饲养层10X补充液,50 mL

总览

可在无bFGF和无饲养层的低氧条件下,将激发态的人胚胎干细胞(ES)和诱导多能干细胞(iPS)回转为类原始态的状态,并维持其类原始态的特征。

该无血清细胞培养基由魏茨曼科学研究所授权开发,可稳定诱导细胞呈现类原始状态特征,如紧密排列、具有折光边缘的圆顶状的克隆形态。使用 RSeT™ 无饲养层培养基培养的人ES/iPS细胞表现出多个关键类原始态标志基因(如 KLF17、KLF2、KLF4 和 TFCP2L1)的表达上调。这些细胞可以进一步分化,也可以通过转入 mTeSR™1 培养体系恢复为激发态后再进行分化。

兼容的分化产品包括:STEMdiff™ 定型内胚层试剂盒(目录号 #05110)、STEMdiff™ SMADi 神经诱导试剂盒(目录号 #08581)和STEMdiff™ 中胚层诱导培养基(目录号 #05220)。

亚型
专用培养基
 
细胞类型
多能干细胞
 
种属

 
应用
细胞培养,扩增,培养
 
品牌
RSeT,TeSR
 
研究领域
干细胞生物学
 
制剂类别
无血清
 

Data Figures

Figure 1. Schematic of Reversion of Primed to Naïve-Like hPSCs with RSeT™ Feeder-Free

Primed hPSCs are plated as aggregates in mTeSR™1. On day 1, mTeSR™1 is replaced with RSeT™ Feeder-Free, the cultures are transferred to hypoxic conditions, and the medium is exchanged every other day. By day 4 or 5, the colonies are generally large enough to be passaged. During the initial culture in RSeT™ Feeder-Free, colonies expand and begin to adopt a tightly-packed, highly domed morphology characteristic of naïve-like stem cells with smooth and refractive colony edges as early as passage 1.

Figure 2. hPSCs Maintained in RSeT™ Feeder-Free are Reverted to a Naïve-Like State and Express High Levels of Naïve-Associated Genes

(A) A representative image of hPSCs that reverted to a naïve-like state after being cultured in RSeT™ Feeder-Free for 1 passage. During reversion, colonies change from a flat morphology to a domed morphology characteristic of naïve-state hPSCs. (B) Expression of naïve-associated genes (KLF2, KLF4, KLF17, TFCP2L1, STELLA, and DNMT3L) in hPSCs that were reverted to a naïve-like state in RSeT™ Feeder-Free. Expression levels were measured by qPCR and normalized to levels in primed hPSCs.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05975
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
05975
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
05975
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

更多信息

更多信息
Species Human
Formulation Category Serum-Free
Legal Statement: Yeda Research and Development Ltd., an Israeli corporation (“YEDA”) is the owner of all right and title to certain patents applications and other proprietary rights which cover (i) the use of the product you are purchasing, such product comprising of media culture compositions to produce novel fully Naïve Induced Pluripotent Stem Cells (“iPSC’s”) (“the Product”) and (ii) the use of the iPSC’s themselves. By purchase of the Product, you shall have, subject to the terms of use below, a limited non-exclusive licence to use the Products to generate iPSC’s and the iPSC’s themselves. YOU HEREBY AGREE TO USE THE PRODUCT AND ANY RESULTS OR PHYSICAL MATTER, INCLUDING IPSC’S, GENERATED FROM THE USE OF THE PRODUCT, OR ANY COPIES OR DERIVATIVES OF ANY OF THE FOREGOING, SOLELY FOR THE PURPOSE OF CONDUCTING NON-COMMERCIAL RESEARCH ACTIVITIES AND PUBLISHING THE RESULTS OF SUCH ACTIVITIES. YOU HEREBY ACKNOWLEDGE THAT ANY USE THEREOF FOR PURPOSES OTHER THAN NON-COMMERCIAL RESEARCH AND PUBLICATION IS PROHIBITED AND SHALL BE PROSECUTED TO THE FULL EXTENT OF THE LAW. IN THE EVENT YOU WISH TO USE THE ABOVE FOR ANY OTHER PURPOSE, A SEPARATE LICENSE IS REQUIRED. YOU CAN CONTACT YEDA AT INFO.YEDA@WEIZMANN.AC.IL FOR THE PURPOSE OF OBTAINING A SEPARATE LICENCE IN THIS REGARD, YEDA BEING IN NO WAY OBLIGATED TO ENTER INTO OR NEGOTIATE SUCH A LICENCE WITH YOU. Nothing contained herein shall be deemed to be a representation or warranty, express or implied, by YEDA that patent applications relating to the Product and the iPSC’s will be granted; (ii) that patents obtained on any of the said patent applications are or will be valid or will afford proper protection; (iii) that the use of the Product or the iPSC’s will not infringe the rights of any third party; or (iv) as to the potential, use, exploitability, merchantability, suitability, effectiveness, fitness for purpose or safety of the Product and the iPSC’s.Under no circumstances shall YEDA be liable for any claims, demands, liabilities, costs, losses, damages or expenses (including legal costs and attorney's fees) of whatever kind (any of the above, a “Loss”) caused to or suffered by any person or entity that directly or indirectly arise out of the use of the Product or the iPSC’s. Copyright © 2017 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, STEMdiff, and RSeT are trademarks of STEMCELL Technologies Canada Inc. mTeSR is a trademark of WARF. All other trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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