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Naïve态人多能干细胞 qPCR 阵列

用于表征与naïve或 primed态 hPSC相关的基因表达
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产品号 #07521_C

用于表征与naïve或 primed态 hPSC相关的基因表达

产品组分包括

  • Naïve态人多能干细胞 qPCR 阵列，Type 1 (产品号 #07521)
      • li>hPSC Naïve State qPCR Array, 1 x 96孔板
    • TBP qPCR Control Template, 1 块
  • Naïve态人多能干细胞 qPCR 阵列，Type 3 (产品号 #07523)
    • hPSC Naïve State qPCR Array, 1 x 96孔板
    • TBP qPCR Control Template, 1 块
  • Naïve态人多能干细胞 qPCR 阵列，Type 5 (产品号 #07525)
    • hPSC Naïve State qPCR Array, 1 x 384孔板
    • TBP qPCR Control Template, 1 块
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总览
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总览

通过精确测量基因表达谱来评估您的人多能干细胞（hPSC），以确定它们在从Naïve到Primed启动多能性谱系的状态。

qPCR 可用于多个样本中基因表达的稳态 mRNA 水平变化，通常以内参基因的相对表达量进行标准化。基因特异性引物扩增由 mRNA 逆转录所得的 cDNA 池中的目标序列；与 TaqMan® 技术类似，序列特异性探针在 Taq DNA 聚合酶的 5′ 外切酶活性作用下产生荧光信号。通过检测荧光信号的累积速率，可定量样本中 cDNA 的含量，从而间接反映原始细胞裂解物中的 mRNA 水平。

人多能干细胞（hPSC）Naïve态 qPCR 阵列配备了经验证的引物和探针，可检测 90 个在naïve 和 primed态 hPSC 中差异表达显著的基因，以及 6 个内源性对照基因。试剂盒还提供 TBP（TATA 结合蛋白）qPCR 对照模板，作为合成 DNA 阳性对照。Naïve态 hPSC 能自我更新，保留着着床前囊胚的特性，并可分化为胚内及胚外谱系；Primed 态 hPSC 同样具备自我更新能力，但其分化潜能仅限于三胚层。更多基因信息及 qPCR 数据分析工具，请访问相应网站。

细胞类型
多能干细胞
 
种属
人
 
应用
基因组编辑
 
研究领域
干细胞生物学
 

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Data Figures

Figure 1. Human Pluripotent Stem Cell Naïve State qPCR Array Plate Configuration

This illustration shows a quadrant of 96 unique primer-probe assays available on a 384-well qPCR plate (A). There are four identical quadrants in total, which together form a complete array of 384 assays used in the Human Pluripotent Stem Cell Naïve State qPCR Array Plate Configuration. The diagram also indicates the wells containing the TBP qPCR control cocktail (H12, H24, P12, P24), which serve as reference wells for assay normalization (B).

Figure 2. Quantitative PCR Analysis of Human Pluripotent Stem Cell Naïve State

Quantitative PCR analysis of gene expression of H1 hESCs cultured in a naive-like state compared to H1 hESCs cultured under a primed state. Genes associated with higher expression in a naive state than a primed state are shown in light green, genes expected to be more highly expressed in a primed state compared to a naive state are shown in orange and pluripotency associated genes expressed highly in both naive and primed states are shown in yellow. Genes associated with endoderm (red), mesoderm (light blue) ectoderm (dark green) and partially reprogrammed genes (dark blue) in feeder-free versus feeder-dependent tissue culture are also show. Y-axis measures the log change expression difference and X-axis indicates the name of the gene. Experiments were run on a Viia7 Fast (Applied Biosystems) Instrument and data analysis was performed using STEMCELL’s qPCR analysis program.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Pluripotent Stem Cell Naïve State qPCR Array
Catalog #
07521, 07525
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human Pluripotent Stem Cell Naïve State qPCR Array
Catalog #
07521
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human Pluripotent Stem Cell Naïve State qPCR Array
Catalog #
07521, 07525
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human Pluripotent Stem Cell Naïve State qPCR Array
Catalog #
07525
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (1)

qPCR Arrays for Cell Characterization
Brochure
qPCR Arrays for Cell Characterization

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Species Human
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