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EpiCult™Plus Medium

用于培养人、小鼠和大鼠上皮干细胞
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产品号 #（选择产品）

产品号 #06070_C

用于培养人、小鼠和大鼠上皮干细胞

产品优势

  • 一种不含血清和bpe的细胞培养基，与传统培养基相比，可促进上皮干细胞的增殖
  • 细胞保持超过30倍的增殖能力而不衰老
  • 维持上皮干细胞，使其能够分化为原组织中存在的细胞类型
  • 在传代和实验之间提供一致的性能

产品组分包括

  • EpiCult™Plus基础培养基，490毫升
  • EpiCult™Plus补充剂，10毫升
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Trypsin-EDTA (0.25%)
    Trypsin-EDTA (0.25%)

    Enzymatic cell dissociation reagent

  2. Hydrocortisone Stock Solution
    Hydrocortisone Stock Solution

    Cell culture supplement

  3. CellAdhereᵀᴹ Collagen I-Coated Plate
    CellAdhereᵀᴹ Collagen I-Coated Plate

    Sterile flat-bottom, tissue culture-treated, collagen I-coated multiwell plate with lid; 6-, 12-, 24-, 48-, 96-well formats

  4. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

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实验数据
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概述

EpiCult™Plus Medium是一种不含血清和bpe的培养基，用于扩增来自人、小鼠和大鼠的乳腺、前列腺和其他分层和假分层上皮组织的上皮干细胞。上皮细胞可在EpiCult™Plus培养基中扩增，可进行多次传代，扩增倍数可达30倍；对于某些人乳腺细胞，可能需要添加胎牛血清。

EpiCult™Plus Medium不推荐用于人角质形成细胞或原代人气道细胞；对于培养原代人气道细胞，我们推荐使用PneumaCult™-Ex Plus Medium（目录#05040）。

注：氢化可的松储备溶液（目录#07925）需要准备完整的EpiCult™Plus培养基。

Subtype
Specialized Media
 
Cell Type
Epithelial Cells
 
Species
Human
 
Application
Cell Culture
 
Brand
EpiCult
 
Area of Interest
Epithelial Cell Biology
 
Formulation Category
Serum-Free
 

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Data Figures

EpiCult™ Plus Enables the Long-Term Growth of Epithelial Stem Cells From Prostate and Mammary Tissues

Figure 1. EpiCult™ Plus Enables the Long-Term Growth of Epithelial Stem Cells From Prostate and Mammary Tissues

(A) Mouse mammary, (B) mouse prostate, and (C) human prostate samples were established and expanded in EpiCult™ Plus. Growth curves from three independent samples are shown here.

Human Mammary Epithelial Cells Expand More Rapidly When Cultured in EpiCult™ Plus Compared to Conventional Epithelial Cell Expansion Media

Figure 2. Human Mammary Epithelial Cells Expand More Rapidly When Cultured in EpiCult™ Plus Compared to Conventional Epithelial Cell Expansion Media

Human mammary epithelial cells expanded in EpiCult™ Plus (solid line, solid markers) can be grown beyond 20 population doublings while cells grown in conventional epithelial cell expansion medium (dotted line, solid markers) senesced after approximately 10 population doublings. The addition of FBS to EpiCult™ Plus (solid line, open markers) allowed further expansion of the human mammary epithelial cells.

Cells Cultured in EpiCult™ Plus are Maintained in a Basal-Like State

Figure 3. Cells Cultured in EpiCult™ Plus are Maintained in a Basal-Like State

EpiCult™ Plus maintains epithelial stem cells in a basal-like state as observed by the marker expression of CD49f in (A) mouse mammary, (B) mouse prostate, (C) human mammary, and (D) human prostate epithelial cells that were cultured in EpiCult™ Plus for a minimum of 8 passages. Flow cytometry analysis shows > 99% expression of CD49f across tissues.

Epithelial Cells Expanded with EpiCult™ Plus can be Differentiated as Organoids

Figure 4. Mouse Prostate and Mammary Epithelial Cells Expanded with EpiCult™ Plus Can Be Differentiated as Organoids

Mouse epithelial cells were expanded in EpiCult™ Plus and differentiated into either (A,B) prostate or (C,D) mammary organoids. (B,D) Both mouse prostate and mammary organoids express the luminal marker keratin 8 (green) and the basal marker keratin 14 (red) as shown by immunofluorescence microscopy; nuclei are counterstained with DAPI. This data demonstrates the compatibility of EpiCult™ Plus with both mouse prostate and mammary workflows that may require the robust upstream expansion of epithelial cells.

Human Prostate Epithelial Cells Expanded in EpiCult™ Plus Can Be Differentiated as Organoids

Figure 5. Human Prostate Epithelial Cells Expanded in EpiCult™ Plus Can Be Differentiated as Organoids

Freshly dissociated human prostate cells were (A) seeded and expanded in EpiCult™ Plus. After 5 days in culture, the cells were harvested and seeded into human prostate organoid medium and maintained for an additional (B) 11 days. (C) The resulting organoids contain a cell-dense core composed of luminal cells expressing keratin 8 (green) and androgen receptors (magenta), while basal cells expressing keratin 14 (red) line the outer side of the prostate organoid. This demonstrates that EpiCult™ Plus supports the robust expansion of human prostate organoid-forming cells.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EpiCult™ Plus Medium
Catalog #
06070
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EpiCult™ Plus Medium
Catalog #
06070
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EpiCult™ Plus Medium
Catalog #
06070
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Human
Formulation Category Serum-Free
Legal Statement: This product was developed under a license to intellectual property owned or controlled by Propagenix, Inc. This product is sold for research use only (which includes pre-clinical research) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes or clinical use. Purchasers wishing to use the product for purposes other than research use should contact Propagenix, Inc. (www.propagenix.com/about#contact-us). PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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