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PneumaCult™气道类器官试剂盒

无血清和无bpe培养基用于气道类器官的高效建立和分化
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产品号 #（选择产品）

产品号 #05060_C

无血清和无bpe培养基用于气道类器官的高效建立和分化

产品优势

  • 三维体外系统，概括了人体内气道的关键特征
  • 用于人气道上皮细胞扩增和分化的完整培养基配方，与PneumaCult™-Ex Plus兼容
  • 方便的格式和易于使用的协议，提供一致的结果

产品组分包括

  • PneumaCult™气道类器官基础培养基，450 mL（目录#05061）
  • PneumaCult™气道类器官播种补充剂，10 mL（目录#05062）
  • PneumaCult™气道类器官分化补充剂，40ml（目录#05063）
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Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. PneumaCult™-Ex Plus Medium
    PneumaCult™-Ex Plus Medium

    Serum- and BPE-free medium for expansion of primary human airway epithelial cells

  2. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  3. Hydrocortisone Stock Solution
    Hydrocortisone Stock Solution

    Cell culture supplement

  4. Heparin Solution
    Heparin Solution

    Cell culture supplement

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

PneumaCult™气道类器官试剂盒是一种不含血清和bpe的培养基系统，用于有效地建立和分化源自人支气管上皮细胞（HBECs）的气道类器官。
在PneumaCult™-Ex Plus培养基（#05040）中进行2D扩展后，PneumaCult™气道类器官种子培养基允许开始3D类器官培养，然后使用PneumaCult™气道类器官分化培养基获得形态学相关的气道类器官。气道类器官为研究气道上皮提供了一种功能性的体外器官型培养系统。当完全分化时，类器官表现为中央管腔，周围是极化的气道上皮细胞层，由分化的细胞类型组成，如纤毛细胞和杯状细胞。气道类器官培养的应用包括肺部疾病建模、药物疗效和毒性筛选以及气道上皮功能研究。

浏览我们的常见问题（faq）关于使用PneumaCult™培养气道类器官的问题。

如果您打算将此产品用于商业用途，请通过www.huborganoids.nl获取商业用途许可证或HUB许可的相关说明。

Subtype
Specialized Media
 
Cell Type
Airway Cells
 
Species
Human
 
Application
Cell Culture, Differentiation, Expansion, Organoid Culture
 
Brand
PneumaCult
 
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Respiratory Research
 
Formulation Category
Serum-Free
 

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Data Figures

Schematic of human airway organoid culture workflow using PneumaCult™ Airway Organoid Kit

Figure 1. Overview of the PneumaCult™ Culture System for Human Airway Organoid Generation

In the early two-dimensional expansion phase of the human airway organoid culture procedure, HBECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs are then embedded into a Matrigel® dome and expanded for 4 - 7 days using PneumaCult™ Airway Organoid Seeding Medium. Following the expansion, the HBECs are differentiated using PneumaCult™ Airway Organoid Differentiation Medium for an additional 21+ days.

Fully differentiated human airway organoids generated using PneumaCult™ Airway Organoid Kit display physiologically relevant morphology.

Figure 2. Fully Differentiated Human Airway Organoids Generated Using PneumaCult™ Airway Organoid Kit

(A) Bright-field image of airway organoids growing in PneumaCult™ Airway Organoid Seeding Medium at day 7 exhibit basal cell spheroid morphology. (B) Bright-field image of airway organoids differentiated in PneumaCult™ Airway Organoid Differentiation Medium at day 21 exhibit hollow lumens. (C) Airway organoid stained for ZO-1 (junction protein marker, red), MUC5AC (goblet cell marker, purple), AC-Tubulin (ciliated cell marker, green), and DAPI (nuclei, blue).

Comparison of Forskolin-treated airway organoids derived from healthy donors and CF donors.

Figure 3. Forskolin-Induced Swelling of Airway Organoids

(A) Forskolin-treated organoids derived from healthy donors increased in size compared to the DMSO control, indicating functional CFTR protein expression. (B) Forskolin-induced swelling is lost in organoids derived from CF donors, but re-established in VX-809-treated airway organoids. Error bars represent ± 95% confidence interval for the mean (n=3). Bright-field images of airway organoids taken during the Forskolin swelling assay at (C) 0 hours and (D) 6 hours show organoid swelling after treatment.

Ciliated cell percentage in fully differentiated airway organoids derived from healthy and cystic fibrosis (CF) donors using PneumaCult™ Airway Organoid Kit at different passages.

Figure 4. Fully Differentiated Airway Organoids Retain Morphological Characteristics at Different Passages

The ciliated cell percentage in organoids grown from (A) healthy and (B) CF donors using PneumaCult™ Airway Organoid Kit increased from P3 to P5. The total and ciliated cells were counted using a hemocytometer. Error bars represent ± 95% confidence interval for the mean (n=3).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
PneumaCult™ Airway Organoid Kit
Catalog #
05060
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Workflow Stages for Organoids
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Resources and Publications

Educational Materials (22)

Pulmonary Research
Brochure
Pulmonary Research
Organoids
Brochure
Organoids
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Protocol
How to Process Epithelial Organoids and Organoid-Derived Epithelial Monolayers for RNA Isolation
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
Protocol
Dissociation of Mouse Lung Tissue into a Single-Cell Suspension
Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors
Protocol
Forskolin-Induced Swelling of Airway Organoids Derived from Healthy and Cystic Fibrosis Donors
Dynamic Modeling in Organoids
Wallchart
Dynamic Modeling in Organoids
Cellular Organization and Biology of the Respiratory System
Wallchart
Cellular Organization and Biology of the Respiratory System
What Are the Impacts of New Legislative Actions on Drug Development?
3:07
Video
What Are the Impacts of New Legislative Actions on Drug Development?
Beating Cilia of Airway Organoids Grown in PneumaCult™
0:30
Video
Beating Cilia of Airway Organoids Grown in PneumaCult™
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Using Stem Cell-Based Models in Drug Discovery
46:49
Webinar
Using Stem Cell-Based Models in Drug Discovery
Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
51:30
Webinar
Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
Human Airway Organoids to Study Host-Pathogen Interactions
35:24
Webinar
Human Airway Organoids to Study Host-Pathogen Interactions
Human Lung Development and Disease: Lessons from Organoids
26:16
Webinar
Human Lung Development and Disease: Lessons from Organoids
In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
18:41
Webinar
In Vitro Human Airway Modeling Using Primary Airway Epithelial Cells
Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
12:13
Webinar
Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
Organoid-Based Assays for Disease Modeling
24:55
Webinar
Organoid-Based Assays for Disease Modeling
Reliable In Vitro Culture Solutions for Your Alveolar Research
18:52
Webinar
Reliable In Vitro Culture Solutions for Your Alveolar Research
Studying Respiratory Viruses with ALI Cultures
6:56
Webinar
Studying Respiratory Viruses with ALI Cultures
Elucidating Parechovirus A Pathogenesis Using Organoid Models
30:09
Webinar
Elucidating Parechovirus A Pathogenesis Using Organoid Models
Dr. Bas Trietsch
Interview
Dr. Bas Trietsch

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Species Human
Formulation Category Serum-Free
Legal Statement: This product was developed under a license to intellectual property owned by Hubrecht Organoid Technology (HUB). This product is sold for research use only. Purchase of this product does not include the right to use it for drug screening aiming for commercial gain, equipment validation, biobanking, or for other commercial purposes. Purchasers wishing to use the product for purposes other than basic research use should contact HUB at www.huborganoids.nl to obtain a further license. Purchasers may apply for a License from HUB, which will not be unreasonably withheld by HUB. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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