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ImmunoCult™小鼠Treg分化补充剂

补充小鼠naïve CD4+ T细胞向调节性T细胞（Tregs）分化
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产品号 #（选择产品）

产品号 #10957_C

补充小鼠naïve CD4+ T细胞向调节性T细胞（Tregs）分化

产品优势

  • 优化了从C57BL/6小鼠脾脏分离的naïve CD4+ T细胞诱导Tregs的方法
  • 作为100X浓缩物供应；解冻和混合后，管内容物可直接添加到介质中
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概述

ImmunoCult™小鼠Treg分化补充剂包含重组小鼠和人类细胞因子的组合以及优化的小分子，可促进小鼠naïve CD4+ T细胞向调节性T细胞（Treg）分化。该补充剂用于含有胎牛血清和其他添加剂的RPMI 1640培养基（目录#36750），以及抗小鼠CD3和抗小鼠CD28单克隆抗体作为活化剂。

本产品推荐用于：
•小鼠naïve T细胞分化和Treg功能调控的研究
•开发将naïve T细胞向Treg亚群扩展和极化的程序

Contains
• Recombinant mouse interleukin 2 (IL-2)
• Recombinant human transforming growth factor beta (TGF-β)
• All-trans retinoic acid (ATRA)
 
Subtype
Supplements
 
Cell Type
T Cells, T Cells, CD4+, T Cells, Regulatory
 
Species
Mouse
 
Application
Cell Culture, Differentiation, Expansion
 
Brand
ImmunoCult
 
Area of Interest
Immunology
 

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Data Figures

Figure 1. ImmunoCult™ Mouse Treg Differentiation Supplement Produces CD4+CD25+FOXP3+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone (Non-polarizing cultures), or in medium supplemented with ImmunoCult™ Mouse Treg Differentiation Supplement (Polarizing cultures) for 6 days. Cells were subsequently stained with anti-CD4, anti-CD25, anti-FOXP3 and a viability dye and analyzed by flow cytometry. Shown is the expression of CD25 and FOXP3 back-gated on viable, CD4+ cells from non-polarized (A) or polarized cultures (B). The mean proportion of CD4+FOXP3+ cells is significantly higher in cells cultured in ImmunoCult™ Mouse Treg Differentiation Supplement (91 ± 2%) compared to non-polarized cells (2 ± 0.4%) (p < 0.001; n = 14). Data from experimental groups were compared using a paired T-test.

Figure 2. ImmunoCult™ Mouse Treg Differentiation Supplement Expands Viable CD4+ Cells Under Treg Polarizing Conditions

Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28 and cultured in medium alone, or medium containing ImmunoCult™ Mouse Treg Differentiation Supplement for 6 days. A 3.6-fold expansion of total nuclear cells (TNC) ensues in cultures polarized with ImmunoCult Mouse Treg Differentiation Supplement (purple column), which is significantly higher compared to non-polarized cultures (grey column). (Data represent the mean ± SEM, n = 14, ***p < 0.001. Data from experimental groups were compared using a paired T-test).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse Treg Differentiation Supplement
Catalog #
10957
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ImmunoCult™ Mouse Treg Differentiation Supplement
Catalog #
10957
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Species Mouse
Contains • Recombinant mouse interleukin 2 (IL-2) • Recombinant human transforming growth factor beta (TGF-β) • All-trans retinoic acid (ATRA)
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to All-trans Retinoic Acid which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
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