切换导航
  • 比较产品
我的购物车

细胞外囊泡SEC柱

用于从生物液体中分离细胞外囊泡的粒径排除色谱柱
率先评论此产品
添加并比较

产品号 #（选择产品）

产品号 #100-0414_C

用于从生物液体中分离细胞外囊泡的粒径排除色谱柱

产品优势

  • 快速，易于使用的协议
  • 可重复使用（最多五次）

产品组分包括

  • 0.5 mL细胞外囊泡SEC柱
    • 10个（目录编号100-0414）
  • 2 mL细胞外囊泡SEC柱
    • 5个（目录编号100-0415）
  • 20 mL细胞外囊泡SEC柱
    • 3台（目录编号100-0416）
main product photo
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. D-PBS (Without Ca++ and Mg++)
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

  2. Costar® Microcentrifuge Tubes
    Costar® Microcentrifuge Tubes

    Non-sterile, clear, polypropylene snap-cap microcentrifuge tubes

总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

概述

用细胞外囊泡大小隔离色谱（SEC）柱从多种生物基质中分离和纯化细胞外囊泡（ev），包括血浆、血清和细胞培养基。与其他EV分离方法相比，有效地将EV从循环蛋白中分离出来，而囊泡的改变（包括功能或大小）最小。SEC柱分离快速简便，随后ev适用于下游分析，如流式细胞术、western blot、核酸提取和/或功能分析。SEC柱有0.5 mL（10列/包），2 mL（5列/包）和20 mL（3列/包）大小，以支持不同的样品体积，可使用多达五次。

Subtype
Size Exclusion Chromatography Columns
 
Species
Human, Mouse, Non-Human Primate, Other, Rat
 
Application
Extracellular Vesicle Isolation
 
Area of Interest
Extracellular Vesicle Research
 

查看更多 显示更少

Data Figures

Graphs showing the fractions in which particles and proteins, isolated from human plasma using the 0.5 mL Extracellular Vesicle SEC Column, were detected by nanoparticle tracking analysis and bicinchoninic acid assays, respectively.

Figure 1. Isolation of Extracellular Vesicles from Plasma Using a 0.5 mL Extracellular Vesicle SEC Column

The 0.5 mL Extracellular Vesicle SEC Column was loaded with 0.5 mL of human plasma. 100 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis (NTA) and bicinchoninic acid (BCA) assays, respectively. EVs were detected in fractions 9 - 14, while proteins were detected in fractions 15 onwards.

Graphs showing the fractions in which particles and proteins, isolated from human plasma using the 2 mL Extracellular Vesicle SEC Column, were detected by nanoparticle tracking analysis and bicinchoninic acid assays, respectively.

Figure 2. Isolation of Extracellular Vesicles from Plasma Using a 2 mL Extracellular Vesicle SEC Column

The 2 mL Extracellular Vesicle SEC Column was loaded with 2 mL of human plasma. 500 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 7 - 10, while proteins were detected in fractions 11 onwards.

Graphs showing the fractions in which particles and proteins, isolated from concentrated serum-free medium conditioned by mesenchymal stromal cell culture, using the 20 mL Extracellular Vesicle SEC Column, were detected by nanoparticle tracking analysis and bicinchoninic acid assays, respectively.

Figure 3. Isolation of Extracellular Vesicles from Conditioned Medium Using a 20 mL Extracellular Vesicle SEC Column

The 20 mL Extracellular Vesicle SEC Column was loaded with 20 mL of 10-fold concentrated serum-free medium conditioned by mesenchymal stromal cell (MSC) culture. 1 mL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 15 - 30, while proteins were detected in fractions 35 onwards. Fractions 31-33, which are outside of the typical EV elution range, may still contain EVs with low protein contamination for serum-free media samples.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
0.5 mL Extracellular Vesicle SEC Columns
Catalog #
100-0414
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
20 mL Extracellular Vesicle SEC Columns
Catalog #
100-0416
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
2 mL Extracellular Vesicle SEC Columns
Catalog #
100-0415
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Species Human, Mouse, Non-Human Primate, Other, Rat
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
版权 © 2025 STEMCELL Technologies 技术有限公司。保留所有权利。