Isolate and purify extracellular vesicles (EVs) from numerous biological matrices, including plasma, serum, and cell culture media, with Extracellular Vesicle Size Exclusion Chromatography (SEC) Columns. Efficiently separate EVs from circulating proteins with minimal vesicle alteration, including functionality or size, compared to other EV isolation methods. SEC Column isolation is quick and easy, following which EVs are suitable for downstream analysis, such as flow cytometry, western blot, nucleic acid extraction, and/or functional assays. SEC Columns are available in 0.5 mL (10 columns/pack), 2 mL (5 columns/pack), and 20 mL (3 columns/pack) sizes to support different sample volumes and can be used up to five times.
Data Figures
Figure 1. Isolation of Extracellular Vesicles from Plasma Using a 0.5 mL Extracellular Vesicle SEC Column
The 0.5 mL Extracellular Vesicle SEC Column was loaded with 0.5 mL of human plasma. 100 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis (NTA) and bicinchoninic acid (BCA) assays, respectively. EVs were detected in fractions 9 - 14, while proteins were detected in fractions 15 onwards.
Figure 2. Isolation of Extracellular Vesicles from Plasma Using a 2 mL Extracellular Vesicle SEC Column
The 2 mL Extracellular Vesicle SEC Column was loaded with 2 mL of human plasma. 500 μL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 7 - 10, while proteins were detected in fractions 11 onwards.
Figure 3. Isolation of Extracellular Vesicles from Conditioned Medium Using a 20 mL Extracellular Vesicle SEC Column
The 20 mL Extracellular Vesicle SEC Column was loaded with 20 mL of 10-fold concentrated serum-free medium conditioned by mesenchymal stromal cell (MSC) culture. 1 mL fractions were collected and analyzed for particle and protein content by nanoparticle tracking analysis and bicinchoninic acid assays, respectively. EVs were detected in fractions 15 - 30, while proteins were detected in fractions 35 onwards. Fractions 31-33, which are outside of the typical EV elution range, may still contain EVs with low protein contamination for serum-free media samples.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
0.5 mL Extracellular Vesicle SEC Columns
Catalog #
100-0414
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
20 mL Extracellular Vesicle SEC Columns
Catalog #
100-0416
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
2 mL Extracellular Vesicle SEC Columns
Catalog #
100-0415
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Extracellular Vesicle Research
Resources and Publications
Educational Materials (4)
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