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抗人CD14抗体克隆M5E2

小鼠抗人、恒河猴、食蟹虫CD14单克隆IgG2a抗体
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产品号 #60004_C

小鼠抗人、恒河猴、食蟹虫CD14单克隆IgG2a抗体

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概述

M5E2抗体与CD14反应，CD14是一种约53 - 55 kDa的gpi锚定跨膜糖蛋白，在外周血单核细胞和巨噬细胞表面高水平表达，在粒细胞表面低水平表达。CD14在单核/巨噬细胞和粒细胞之间的表达水平相差约10倍，这使得CD14成为区分这些细胞群的有用标记物。CD14也存在于组织巨噬细胞、朗格汉斯细胞和树突状细胞中。CD14是脂多糖（LPS）复合物和血清脂多糖结合蛋白的高亲和力受体，并通过作为tlr4和MD-2的共受体，在革兰氏阴性病原体的免疫反应中调节脂多糖依赖的信号转导。这触发NF-kappa-B的激活、细胞因子的分泌和炎症反应的诱导。CD14的两种可溶形式也被描述（~48和~55 kDa）。

该抗体克隆已通过EasySep™试剂盒分离细胞的纯度评估验证，包括EasySep™人髓系阳性选择试剂盒（目录#18653）和EasySep™人CD14阳性选择试剂盒（目录#18058）；可以观察到部分阻断，以及EasySep™HLA全血CD33阳性选择试剂盒（目录#18287HLA）， EasySep™HLA全血髓系阳性选择试剂盒（目录#18683HLA）和EasySep™人黄褐色毛CD14阳性选择试剂盒（目录#18088）。

Subtype
Primary Antibodies
 
Target Antigen
CD14
 
Alternative Names
LPS receptor
 
Reactive Species
Capuchin Monkey, Chimpanzee, Common Marmoset, Cotton-Topped Tamarin, Cow, Cynomolgus, Dog, Human, Macaque, Pig, Rhesus, Sheep, Squirrel Monkey
 
Conjugation
Alexa Fluor 488, APC, Biotin, FITC, PE, Unconjugated
 
Host Species
Mouse
 
Cell Type
Monocytes
 
Species
Human, Non-Human Primate, Other
 
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neutralization and Blocking
 
Area of Interest
Immunology
 
Clone
M5E2
 
Gene ID
929
 
Isotype
IgG2a, kappa
 

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Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488 and Anti-Human CD45 Antibody, Clone HI30, APC (Catalog #60018AD). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 is shown (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, APC (filled histogram) or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC (Catalog #60071AZ; solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, APC is shown (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin (Catalog #60071BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by SAV APC is shown (solid line histogram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Biotin followed by SAV APC is shown (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC (Catalog #60071FI; solid line histogram). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human Myeloid Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC is shown (solid line historgram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, FITC is shown (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, PE (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone HI30, APC (Catalog #60018AZ). (B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD14 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, PE. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) is shown (solid line historgram). (C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood CD33 Positive Selection Kit and labeled with Anti-Human CD14 Antibody, Clone M5E2, FITC. Histograms show labeling of whole blood nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE is shown (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD14 Antibody, Clone M5E2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, PE
Catalog #
60004PE.1, 60004PE
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2
Catalog #
60004
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, APC
Catalog #
60004AZ, 60004AZ.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488
Catalog #
60004AD, 60004AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, FITC
Catalog #
60004FI, 60004FI.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, Biotin
Catalog #
60004BT
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, PE
Catalog #
60004PE.1, 60004PE
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2
Catalog #
60004
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, APC
Catalog #
60004AZ, 60004AZ.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, Alexa Fluor® 488
Catalog #
60004AD, 60004AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, FITC
Catalog #
60004FI, 60004FI.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human CD14 Antibody, Clone M5E2, Biotin
Catalog #
60004BT
Lot #
All
Language
English

更多信息

更多信息
Species Human, Non-Human Primate, Other
Clone M5E2
Gene Id 929
Alternative Names LPS receptor
Isotype IgG2a, kappa
Legal Statement: Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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