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鬼笔环肽iFluor™偶联物

适用于细胞培养中F-肌动蛋白检测的荧光染料偶联物
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产品号 #（选择产品）

产品号 #100-0852_C

适用于细胞培养中F-肌动蛋白检测的荧光染料偶联物

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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
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总览

鬼笔环肽是一种七肽，来源于有毒真菌毒蝇伞（Amanita phalloides）。鬼笔环肽可高特异性地结合细胞内的F-肌动蛋白（F-actin）丝，并可防止其去聚合。荧光标记的鬼笔环肽可用于对经甲醛固定并通透处理后的细胞中F-actin的标记、识别与定量分析。与大多数动植物物种的肌动蛋白抗体相比，荧光鬼笔环肽表现出更低的非特异性结合，从而在成像过程中提供较低背景信号和更高图像对比度。

细胞类型
神经细胞，PSC衍生，神经元，多能干细胞
 
应用
鉴定
 
研究领域
癌症，神经科学，干细胞生物学
 
CAS 编号
67-68-5
 
分子量
鬼笔环肽，iFluor™ 488*: 1399.49 克/摩尔; 鬼笔环肽，iFluor™ 555*: 1468.53 克/摩尔; 鬼笔环肽，iFluor™ 647: 1632.87 克/摩尔
 

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Data Figures

Image of H9 embryonic stem cells stained with Phalloidin-iFluor™ 647.

Figure 1. Phalloidin-iFluor™ Can Be Used to Stain Embryonic Stem Cells

H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).

Image of forebrain-type neurons stained with Phalloidin-iFluor™ 647.

Figure 2. Phalloidin-iFluor™ Can Be Used to Stain Forebrain-Type Neurons

WLS-1C cell-derived neural progenitor cells were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation (Catalog #08600) and STEMdiff™ Forebrain Neuron Maturation Kits (Catalog #08605). Neurons were cultured on poly-L-ornithine/laminin-coated 35 mm glass-bottom dishes for 3 weeks, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 488
Catalog #
100-0852
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 647
Catalog #
100-0854
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Phalloidin, iFluor™ 555
Catalog #
100-0853
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 488
Catalog #
100-0852
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 647
Catalog #
100-0854
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Phalloidin, iFluor™ 555
Catalog #
100-0853
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

更多信息

更多信息
Molecular Weight Phalloidin, iFluor™ 488*: 1399.49 g/mol; Phalloidin, iFluor™ 555*: 1468.53 g/mol; Phalloidin, iFluor™ 647: 1632.87 g/mol
Cas Number 67-68-5
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