LysoBrite™ dyes are fluorescent dyes that selectively stain lysosomes and other acidic organelles. Due to their hydrophobic nature, LysoBrite™ dyes permeate live cells and selectively accumulate in the lysosomes via the lysosome’s pH gradient. The fluorescence property of these dyes significantly increases within the lysosome’s acidic environment; this fluorescence can be measured using flow cytometry and fluorescence imaging. Due to their selective fluorescence inside lysosomes, LysoBrite™ dyes provide low background and high contrast during imaging. They can be used with proliferating and non-proliferating cells, and are suitable for suspension and adherent cells.
Data Figures
Figure 1. LysoBrite™ Lysosome Dyes Can Be Used to Stain Embryonic Stem Cells
H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then stained with LysoBrite™ Lysosome Dye, Green (green), and counterstained with Hoechst 33342 (blue).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
LysoBrite™ Lysosome Dye, Deep Red
Catalog #
100-0819
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
LysoBrite™ Lysosome Dye, Green
Catalog #
100-0851
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
LysoBrite™ Lysosome Dye, Red DND-99
Catalog #
100-0850
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
LysoBrite™ Lysosome Dye, Deep Red
Catalog #
100-0819
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
LysoBrite™ Lysosome Dye, Green
Catalog #
100-0851
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
LysoBrite™ Lysosome Dye, Red DND-99
Catalog #
100-0850
Lot #
All
Language
English
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