产品号 #72182_C
MEK/ERK通路抑制剂；抑制MEK
总览
PD0325901 是一种选择性、可穿透细胞的 MEK/ERK 通路抑制剂，可抑制 MEK 的激活及其下游信号传导。它是一种强效抑制剂，可在极低浓度（IC50 = 0.33 nM）下抑制 C26 细胞中 ERK 的磷酸化（Bain et al., Barrett et al.）。
维持和自我更新
·与 CHIR99021 联合使用，在没有 LIF 的情况下维持小鼠胚胎干细胞 (ES) 的分化 （Ying et al.）。
·可用于大鼠 ES 细胞的衍生和维持（Buehr et al., Li P et al.）。
重编程
·在重编程后期添加，以筛选和扩增完全重编程的小鼠诱导多能 (iPS) 细胞 （Shi et al., Silva et al.）。
·与 SB431542 和 Thiazovivin 联合使用，可提高人体细胞重编程为iPS细胞的效率（Lin et al.）。
·仅使用单一因子 OCT4 即可促进人体细胞重编程为iPS细胞（Zhu et al.）。
·与 CHIR99021 和 A83-01 联合使用，可从人和大鼠体细胞中生成 mouse-like 或“ground state” iPS细胞（Li W et al.）。
细胞类型
多能干细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
培养，重编程
研究领域
干细胞生物学
CAS 编号
391210-10-9
化学式
C₁₆H₁₄F₃IN₂O₄
纯度
≥98%
通路
MEK/ERK
靶点
MEK
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (4)
Publications (10)
Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell stem cell 2010 DEC
Abstract
A chemical platform for improved induction of human iPSCs. Nature methods 2009 NOV
Abstract
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell stem cell 2009 JAN
Abstract
更多信息
| Species | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 391210-10-9 |
| Chemical Formula | C₁₆H₁₄F₃IN₂O₄ |
| Purity | ≥ 98% |
| Target | MEK |
| Pathway | MEK/ERK |
相关产品
-
ReproRNA™-OKSGM一种非整合型、自我复制的 RNA 重编程载体，用于生成诱导多能干细胞
-
CHIR99021WNT 通路激活剂；抑制 GSK3
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
