PD0325901 is a selective, cell permeable inhibitor of the MEK/ERK pathway that inhibits the activation and downstream signaling of MEK. It is an extremely potent inhibitor, suppressing the phosphorylation of ERK in C26 cells at very low concentrations (IC₅₀ = 0.33 nM) (Bain et al., Barrett et al.).
MAINTENANCE AND SELF-RENEWAL
· Maintains undifferentiated mouse embryonic stem (ES) cells, in combination with CHIR99021, in the absence of LIF (Ying et al.).
· Allows derivation and maintenance of rat ES cells (Buehr et al., Li P et al.).
REPROGRAMMING
· Add at the later stages of reprogramming to select for and expand fully reprogrammed mouse induced pluripotent (iPS) cells (Shi et al., Silva et al.).
· Increases the efficiency of reprogramming human somatic cells to iPS cells, in combination with SB431542 and Thiazovivin (Lin et al.).
· Promotes reprogramming of human somatic cells to iPS cells using only a single factor, OCT4 (Zhu et al.).
· Generates mouse-like or “ground state” iPS cells from human and rat somatic cells, in combination with CHIR99021 and A83-01 (Li W et al.).
MAINTENANCE AND SELF-RENEWAL
· Maintains undifferentiated mouse embryonic stem (ES) cells, in combination with CHIR99021, in the absence of LIF (Ying et al.).
· Allows derivation and maintenance of rat ES cells (Buehr et al., Li P et al.).
REPROGRAMMING
· Add at the later stages of reprogramming to select for and expand fully reprogrammed mouse induced pluripotent (iPS) cells (Shi et al., Silva et al.).
· Increases the efficiency of reprogramming human somatic cells to iPS cells, in combination with SB431542 and Thiazovivin (Lin et al.).
· Promotes reprogramming of human somatic cells to iPS cells using only a single factor, OCT4 (Zhu et al.).
· Generates mouse-like or “ground state” iPS cells from human and rat somatic cells, in combination with CHIR99021 and A83-01 (Li W et al.).
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (4)
Publications (10)
Reprogramming of human primary somatic cells by OCT4 and chemical compounds. Cell stem cell 2010 DEC
Abstract
A chemical platform for improved induction of human iPSCs. Nature methods 2009 NOV
Abstract
The slow kinetics and low efficiency of reprogramming methods to generate human induced pluripotent stem cells (iPSCs) impose major limitations on their utility in biomedical applications. Here we describe a chemical approach that dramatically improves (200-fold) the efficiency of iPSC generation from human fibroblasts, within seven days of treatment. This will provide a basis for developing safer, more efficient, nonviral methods for reprogramming human somatic cells.
Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell stem cell 2009 JAN
Abstract
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