产品号 #05985_C
小鼠骨骼肌祖细胞（卫星细胞）扩增的含血清补充物
总览
MyoCult™扩增添加物10X（小鼠）需与DMEM/F-12联合使用，以配制成MyoCult™扩增培养基，用于培养小鼠骨骼肌祖细胞（卫星细胞）。
该扩增培养基经过优化，适用于扩增经流式细胞分选（FACS）分离的小鼠骨骼肌卫星细胞，也适用于悬浮培养单根肌纤维。在MyoCult™扩增培养基中培养的卫星细胞可进一步分化为多核肌管细胞。
在配制MyoCult™扩增培养基时，除了使用MyoCult™扩增添加物10X，还需加入含15 mM HEPES的DMEM/F-12（产品号 #36254）。
此外，为实现卫星细胞的高效扩增，推荐使用Corning Matrigel®基质（Corning 产品号 #356234）包被的培养皿。
亚型
专用培养基
细胞类型
肌源干/祖细胞
种属
小鼠
应用
细胞培养，扩增
品牌
MyoCult
研究领域
干细胞生物学
Data Figures
Figure 1. FACS-Isolated Pax7+ Mouse Satellite Cells Cultured in MyoCult™ Expansion Medium Expand More Efficiently Than in Homemade Medium
FACS-isolated CD45-/CD31-/Sca1-, alpha7-integrin+/Vcam1+ satellite cells were seeded at 2000 cells/well (24-well plate) and culture-expanded using complete MyoCult™ Expansion Medium (Mouse) or a commonly used homemade medium. Following 6 days of culture, (A) satellite cells were immunostained for nuclei (DAPI, blue) and Pax7 (red). Also, (B) total number and (C) percentage of Pax7+ satellite cells were quantified (n = 3). Error bars represent standard error of mean (SEM). Homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia.
Figure 2. FACS-Isolated Mouse Satellite Cells Are Expandable Over Multiple Passages in Myocult™ Expansion Medium
FACS-isolated CD45-/CD31-/Sca1-, alpha7-integrin+/Vcam1+ satellite cells were seeded at 2000 cells/well (24-well plate) and cultured using complete MyoCult™ Expansion Medium (Mouse) or a commonly used homemade medium. Following 12 days of expansion (2 passages), (A) satellite cells were imaged using phase contrast microscopy and (B) total numbers of MyoD+ satellite cells were quantified (n = 3). Error bars represent SEM. Homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia.
Figure 3. Mouse Satellite Cells Expanded in Myocult™ Expansion Medium Maintain Differentiation Capacity
FACS-isolated CD45-/CD31-/Sca1-, alpha7-integrin+/Vcam1+ satellite cells were culture-expanded using complete MyoCult™ Expansion Medium (Mouse) or a commonly used homemade medium for 2 passages (12 days following FACS isolation) and then treated with differentiation medium (high glucose DMEM with 2% Horse Serum). Following 4 days of differentiation, (A) myotubes cultured in MyoCult™ Expansion Medium were immunostained for nuclei (DAPI, blue) and Myosin Heavy Chain (MyHC, red), and (B) percentage of nuclei localized within MyHC+ myotubes (fusion index) was quantified (n = 3). Error bars represent SEM. Homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia.
Figure 4. Mouse Single Isolated Myofibers Can Be Cultured in MyoCult™ Expansion Medium Without Requiring Additional Supplements
Single isolated myofibers were cultured in suspension using complete MyoCult™ Expansion Medium (Mouse) or a homemade myofiber culture medium. After 4 days, (A) intact, viable myofibers were quantified (n = 3) and (B) immunostained for Pax7 (Red). Error bars represent SEM. Myofiber homemade medium was provided by the lab of Dr. Fabio Rossi, University of British Columbia. A specific myofiber medium (or additional supplements) is not needed for myofiber culture when using MyoCult™ Expansion Medium.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
MyoCult™ Expansion 10X Supplement (Mouse) Catalog #
05985 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
MyoCult™ Expansion 10X Supplement (Mouse) Catalog #
05985 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for Myogenic Stem and Progenitor Cell Research
Workflow Stages for Cardiac and Skeletal Muscle Research
Resources and Publications
Educational Materials (5)
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