产品号 #100-1337_C
干扰素β
干扰素β (IFNβ) 是属于 1 型干扰素家族的一种细胞因子，可与 IFN α/β 受体 (IFNAR) 结合，从而激活酪氨酸激酶并启动干扰素诱导的 Jak-STAT 信号通路，从而调节许多关键的免疫过程 (Smieja et al.)。从大鼠心脏分选成纤维细胞的实验模型中，发现 IFNβ 通过激活不同的 STAT 蛋白来诱导促炎和抗炎细胞因子的产生 (Bolivar et al.)。IFNβ 的抗炎作用已在自身免疫性疾病的背景下得到研究，目前有多种 IFNβ 药物获批用于治疗复发型多发性硬化症 (Filipi 和 Jack)。IFNβ 由免疫细胞，包括巨噬细胞和非免疫细胞，例如成纤维细胞和上皮细胞产生 (Ivashkiv 和 Donalin)。 IFNβ 的晶体结构与其他 I 型干扰素具有共同的特征。它包含五个 α 螺旋，其中四个形成一个螺旋束，以及一个长环和三个短环连接这些螺旋（Karpusas et al.）。为了确保您的应用一致性和可重复性，STEMCELL 的干扰素 α1 采用冻干工艺，纯度≥ 87%，比活 EC50 ≤ 18 pg/mL，并经 LAL 分析验证，确保内毒素水平≤ 1.0 EU/μg 蛋白。
Data Figures
(A) The biological activity of Mouse Recombinant IFN beta was tested by its ability to promote the viability of L929 cells infected with VSV in a cytopathic effect (CPE) assay. Cell viability was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell survival is at 50% of maximum. The EC50 in the above example is ≤18 pg/mL. (B) Mouse Recombinant IFN beta was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IFN beta has a predicted molecular mass of 20.3 kDa and an apparent molecular mass of 30 kDa due to glycosylation.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Product Information Sheet Product Name
Mouse Recombinant IFN beta Catalog #
100-1337 Lot #
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Mouse Recombinant IFN beta Catalog #
100-1337 Lot #
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更多信息
| Species | Mouse |
|---|---|
| Alternative Names | IFB, IFNB |
| Purity | ≥ 87% |
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