产品号 #100-0995_C
选择性地在线粒体中积累的荧光疏水染料
概述
用线粒体追踪染料，深红色有效地标记增殖细胞和非增殖细胞中的线粒体。这种可固定的荧光染料使用快速染色方案，非常适合高通量筛选，并与荧光显微镜兼容。线粒体追踪染料，深红使用标记协议与最少的实践时间，同时促进光稳定性和长保留时间，以提高染色效率。
特点:
•激发波长：640nm
•发射波长：659nm
•由于疏水性，容易穿过活细胞膜
部分依赖于线粒体膜电位
•细胞保留组产生更长的保留时间
•验证用于荧光显微镜
线粒体追踪染料，深红色是一种疏水染料，在线粒体中积累，部分依赖于线粒体膜电位。去极化后，染料重新定位到细胞质中，变得非特异性，但仍保持其红色荧光。线粒体追踪染料含有硫代氯甲基，一种细胞保持基团（Clutton等）。细胞术A， 2019)。氯甲基通过在固定后长时间保持线粒体中的荧光染料来提高染色效率（Clutton等）。细胞术A， 2019)。由于其鲜艳的远红色荧光，线粒体追踪染料，深红适合于多标记实验，如微孔板测定和流式细胞术。使用线粒体追踪染料定量线粒体活性、定位和丰度。
Application
Flow Cytometry
CAS Number
67-68-5
Molecular Weight
~700 g/mol
Data Figures
Figure 1. Human Embryonic Stem Cells Stained with Mitochondrial Tracking Dye, Deep Red
Human embryonic stem cells were cultured using mTesR™ Plus (Catalog #100-0276) on Matrigel®-coated glass-bottom dishes for 24 hours, then labeled with Mitochondrial Tracking Dye, Deep Red (red), and counterstained with Hoechst 33342 (blue).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mitochondrial Tracking Dye, Deep Red Catalog #
100-0995 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Mitochondrial Tracking Dye, Deep Red Catalog #
100-0995 Lot #
All Language
English Applications
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更多信息
| Molecular Weight | ~700 g/mol |
|---|---|
| Cas Number | 67-68-5 |
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