产品号 #100-0991_C
检测线粒体中超氧化物产生的敏感荧光染料
概述
使用线粒体超氧化物染料监测活细胞线粒体中超氧化物的产生。这种荧光染料是检测线粒体超氧化物产生以研究氧化应激的有价值的工具，并且已被证实可用于流式细胞术检测。活性氧（Reactive oxygen species， ROS）和活性氮（Reactive nitrogen species， RNS）是由氧和氮分子产生的自由基。
特点:
•激发波长：510 nm
•发射波长：580 nm
•容易被超氧化物氧化，但不易被ROS或RNS氧化
•氧化产物在细胞中高度荧光
•快速选择性靶向线粒体
•高信噪比
•验证检测使用流式细胞术
线粒体超氧化物染料选择性地靶向活细胞的线粒体。这种染料在与超氧化物（ROS）反应时被氧化，产生红色荧光。在没有超氧化物的情况下，没有吸收，荧光可以忽略不计。线粒体超氧化物染料虽然容易被超氧化物氧化，但对其他ROS和RNS的氧化具有抗性。高信噪比使线粒体超氧化物染料成为细胞呼吸等生物系统中超氧化物检测的合适试剂（Li等）。Front immuno1, 2018)。
Application
Flow Cytometry
Data Figures
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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