产品号 #100-0990_C
用于从哺乳动物细胞或组织中分离富集线粒体亚细胞部分的试剂盒
总览
使用线粒体分离试剂盒，可以便捷地从哺乳动物细胞或组织中富集线粒体和细胞质亚细胞组分。此试剂盒提供经Western blot分析验证的快速线粒体分离技术，适用于研究细胞凋亡及两组分间的信号传导事件。
特点：
快速高效地富集具有活性的线粒体亚细胞组分。
通过蛋白质印迹分析验证线粒体分离效果。
线粒体分离试剂盒提供两种线粒体亚组分分离方案：第一种为基于试剂的方法，可同时处理多达6个样本；第二种采用Dounce匀浆法，可使单个样本的线粒体得率提高两倍。两种方法均通过微量离心技术实现线粒体组分与胞浆组分的分离。
经富集的线粒体组分保持生物活性，可兼容多种下游应用，包括：线粒体呼吸功能研究（Itahana & Zhang Cancer Cell, 2008）、细胞凋亡检测、线粒体内膜电位测定及蛋白质谱分析等。
应用
Western印迹
Data Figures
Figure 1. Western Blot of Mitochondrial Markers After Mitochondrial Isolation from Cultured Cells
Mitochondria were isolated from HEK293 cells with Mitochondrial Isolation Kit using Dounce homogenization. Mitochondrial (M) and cytosolic (C) fractions were analyzed via western blot for two mitochondrial proteins: voltage-dependent anion channels (VDAC) and cytochrome C. Results demonstrate that mitochondria remain intact after enrichment.
Figure 2. Mitochondria Isolated Using the Mitochondrial Isolation Kit Maintain Healthy Mitochondrial Function
Mitochondria were isolated from HEK293 cells using Mitochondrial Isolation Kit and stained with JC-1 (Iodide) dye (Catalog #100-0993) in the presence (grey bar) or absence (orange bar) of valinomycin. High levels of fluorescence indicate that healthy mitochondrial function is retained after isolation.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Mitochondrial Isolation Kit Catalog #
100-0990 Lot #
All Language
English Document Type
Safety Data Sheet 1 Product Name
Mitochondrial Isolation Kit Catalog #
100-0990 Lot #
All Language
English Document Type
Safety Data Sheet 2 Product Name
Mitochondrial Isolation Kit Catalog #
100-0990 Lot #
All Language
English Applications
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