产品号 #100-0993_C
荧光亲脂染料用于量化线粒体膜电位和评估细胞活力
概述
用JC-1（碘化物）染料区分活细胞线粒体膜电位高低。JC-1提供了一种快速方便的染色方案，非常适合高通量筛选，并与流式细胞术应用兼容。
特点:
•激发波长：515nm
•发射波长：530纳米
•消光系数：195,000 cm⁻¹M⁻
•基于染料浓度的荧光
•验证与流式细胞术使用
JC-1是一种亲脂性阳离子碳菁染料。这种染料可以可逆地改变其荧光，这取决于其单体和聚合形式之间的平衡。JC-1以单体形式存在于极化线粒体中，激发后发出最大发射波长为530 nm的绿色荧光。在较高浓度下，由于线粒体膜电位高，j聚集体形成并发出最大发射波长为590 nm的绿橙色荧光。JC-1既可以定性地测量荧光发射的位移，也可以定量地检测FL1通道（FITC）和FL2通道（PE）中荧光强度的差异。
绿色与橙色荧光强度比用于评估细胞凋亡过程中线粒体去极化的发生（Sivandzade等）。Bio-protocol, 2019)。JC-1已被用于同时评价髓系白血病细胞的耐药参数p -糖蛋白（P-gp）活性和凋亡（Chaoui等）。B临床细胞术，2006)。
Alternative Names
5,5’,6,6’-Tetrachloro-1,1’,3,3’-tetraethylbenzimidazolylcarbocyanine iodide; 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide; CBIC2(3); JC-1
Application
Flow Cytometry
CAS Number
3520-43-2
Molecular Weight
652.23 g/mol
Data Figures
Figure 1. Mitochondrial Depolarization Is Visualized by a Shift to JC-1 (Iodide) Monomers
Flow cytometry analysis of Jurkat cells labeled with JC-1 (Iodide) after incubation in the presence or absence of staurosporine, an inducer of apoptosis. Mitochondrial depolarization is visualized by the population shift from JC-1 aggregates to JC-1 monomers after treatment with staurosporine.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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JC-1 (Iodide) Catalog #
100-0993 Lot #
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更多信息
| Molecular Weight | 652.23 g/mol |
|---|---|
| Alternative Names | 5, 5’, 6, 6’-Tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide; 5, 5′, 6, 6′-Tetrachloro-1, 1′, 3, 3′-tetraethyl-imidacarbocyanine iodide; CBIC2(3); JC-1 |
| Cas Number | 3520-43-2 |
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