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ImmunoCult™ Mouse B Cell Expansion Kit

Serum-free culture kit for in vitro expansion of mouse B cells.
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产品号 #100-1003_C

Serum-free culture kit for in vitro expansion of mouse B cells.

产品组分包括

  • ImmunoCult™-XF B Cell Base Medium, 100 mL (Catalog #100-0646)
  • ImmunoCult™-ACF Mouse B Cell Expansion Supplement, 2 mL (Catalog #100-1004)
main product photo
ImmunoCult™ Mouse B Cell Expansion Kit
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

Consistently expand mouse B cells in serum-free conditions without the use of problematic feeder cells.

Use ImmunoCult™ Mouse B Cell Expansion Kit to provide optimized culture conditions for expanding B cells from mouse splenocytes with high yields. This kit comprises ImmunoCult™-XF B Cell Base Medium and ImmunoCult™ACF Mouse B Cell Expansion Supplement, providing you with a robust and easy-to-use culture system. After just 6 - 9 days in culture, cells can be harvested and used directly in your downstream applications.

This kit is compatible with our other upstream and downstream products. For example, you can isolate B cells with EasySep™ Mouse Pan-B Cell Isolation Kit and expand them immediately using ImmunoCult™ Mouse B Cell Expansion Kit.

Subtype
Specialized Media
 
Cell Type
B Cells
 
Species
Mouse
 
Application
Cell Culture, Expansion
 
Brand
ImmunoCult
 
Area of Interest
Antibody Development, Immunology, Infectious Diseases, Cell Therapy Development
 
Formulation Category
Animal Component-Free, Serum-Free, Xeno-Free
 

Data Figures

Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

Figure 1. Protocol Diagram for Culturing Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured in complete Mouse B Cell Expansion Medium as described in Directions For Use (steps 1 - 7). B Cells were harvested on day 9 for analysis. Cells can also be harvested at earlier time points depending on different applications.

Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

Figure 2. Expansion of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Fold expansion of viable cells is shown with bar graphs representing the mean ± SEM (n = 8). B cells expanded 176.9 ± 29.8-fold after 9 days of culture.

Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

Figure 3. Maturation of Mouse B Cells with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Following staining using the protocol by Pracht et al. (Eur J Immunol, 2017), the expression of A) B220 and CD138 and B) TACI (CD267) and CD86 were analyzed by flow cytometry at several time points (data represents mean ± SEM, n = 8). An increase in CD86 cell surface expression indicates B cell activation; a decrease in B220 and an increase in CD138 and TACI cell surface expression indicate maturation of B cells to plasmablasts or plasma cells.

Growth of Mouse B Cells When Cultured with ImmunoCult™ Mouse B Cell Expansion Kit Over the Span of 9 Days

Figure 4. Robust Growth of Mouse B Cells when Cultured with ImmunoCult™ Mouse B Cell Expansion Kit

B cells isolated from mouse spleen using EasySep™ Mouse Pan-B Cell Isolation Kit were cultured as described in Figure 1. Cells were imaged at A) 10X magnification on day 0, B) 10X magnification on day 3, C) 40X magnification on day 6, and D) 40X magnification on day 9.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
ImmunoCult™ Mouse B Cell Expansion Kit
Catalog #
100-1003
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
ImmunoCult™ Mouse B Cell Expansion Kit
Catalog #
100-1003
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
ImmunoCult™ Mouse B Cell Expansion Kit
Catalog #
100-1003
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Legal Statement: Copyright © 2022 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, EasySep, and ImmunoCult are trademarks of STEMCELL Technologies Canada Inc. All other trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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