产品号 #10977_C
人naïve CD4+ T细胞向调节性T细胞（Tregs）分化的无血清培养补充物
概述
ImmunoCult™Human Treg Differentiation Supplement含有一种人类细胞因子和小分子，用于促进外周血源性naïve CD4+人T细胞向调节性T细胞（Treg）的强大激活、扩增和分化。ImmunoCult™人Treg分化补充剂用于与ImmunoCult™-XF T细胞扩增培养基和ImmunoCult™人CD3/CD28 T细胞激活剂一起使用。
本产品推荐用于：
•人类naïve T细胞分化和Treg功能调控的研究
•开发将naïve T细胞扩增和极化为treg的程序
Contains
• Recombinant human TGF-β1
• All-trans retinoic acid
Subtype
Supplements
Cell Type
T Cells, T Cells, CD4+
Species
Human
Application
Cell Culture, Differentiation, Expansion
Area of Interest
Immunology
Data Figures
Figure 1. ImmunoCult™ Human Treg Differentiation Supplement Produces Differentiated CD4+CD45RA-CD45RO+ Cells Under Treg Polarizing Conditions
Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555). Cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Treg Differentiation Supplement (Catalog #10977), cultured in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 days. Shown are flow cytometry results of cell samples stained and analyzed for expression of CD45RA and CD45RO (A) before and (B) after 7 days of culture. After 7 days the majority of Treg cells in culture display a memory T cell phenotype (CD45RA-CD45RO+).
Figure 2. ImmunoCult™ Human Treg Differentiation Supplement Produces Similar Numbers of Differentiated CD4+CD45RA-CD45RO+ Cells Under Treg Polarizing Conditions Than Competitor
Naïve CD4+ T cells were isolated from human peripheral blood samples using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Catalog #19555) and cultured with ImmunoCult™ Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™ Human Treg Differentiation Supplement (Catalog #10977) in ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) (purple), or competitor medium and activator (grey) for 5 days (based on the length of the competitor protocol). The percentage of naïve (CD45RA+CD45RO-) and differentiated (CD45RA-CD45RO+) cells after culture was compared after staining cells for CD45RA and CD45RO and analysis by flow cytometry. The percentage of cells expressing a naïve phenotype and the percentage of cells expressing a differentiated phenotype were not significantly higher with ImmunoCult™ than with competitor medium and activator. Shown are the differences in the percentage of naïve and differentiated T cells in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4).
Figure 3. ImmunoCult™ Human Treg Differentiation Supplement Produces FOXP3+ Cells Under Treg Polarizing Conditions
Naïve CD4+ T cells were cultured for 7 days in ImmunoCult™ activator, supplement and medium using the protocol described in Figure 2. Cells were stained for CD4, CD4, CD25 and FOXP3 and analyzed on days 0, 4, 5 and 7 by flow cytometry. Each bar represents the mean percentage of CD3+CD4+ cells expressing both CD25 and FOXP3 (CD3+CD4+CD25+FOXP3+) (mean ± S.E.M; n = 4 – 20 donors).
Figure 4. Culture with ImmunoCult™ Human Treg Differentiation Supplement Produces Similar Percentages of CD3+CD4+CD25+FOXP3+ Cells as Culture Under Competitor Conditions
Naïve CD4+ T cells were cultured for 5 days in ImmunoCult™ activator, supplement and medium, or competitor medium and activator using the protocol described in Figure 2. The percentage of CD25+FOXP3+ after 5 days of culture was compared after staining cells for CD3, CD4, CD25 and FOXP3 and analysis by flow cytometry. Similar percentages of CD3+CD4+ cells generated with ImmunoCult™ expressed CD25 and FOXP3 as compared to those generated under competitor conditions. Shown are the differences in the percentage of CD3+CD4+ cells expressing both CD25 and FOXP3 (CD3+CD4+CD25+FOXP3+) in either ImmunoCult™ (purple) and competitor (grey) culture conditions (mean ± S.E.M.; paired t-test on logit transformed data; n = 4 - 20).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
ImmunoCult™ Human Treg Differentiation Supplement Catalog #
10977 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
ImmunoCult™ Human Treg Differentiation Supplement Catalog #
10977 Lot #
All Language
English Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Educational Materials (5)
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更多信息
| Species | Human |
|---|---|
| Contains | • Recombinant human TGF-β1 • All-trans retinoic acid |
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE. Safety Statement: CA WARNING: This product can expose you to All-trans Retinoic Acid which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov
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