产品号 #03813_C
筛选转染的原核和真核细胞的抗生素
潮霉素B是一种氨基糖苷类抗生素,能抑制原核和真核细胞的生长。具体来说,它通过干扰70S核糖体亚基的易位和诱导mRNA模板的误读来抑制蛋白质合成。潮霉素B已被用于选择多种细胞中的转染物,包括细菌、原生动物、酵母、真菌、植物和哺乳动物细胞。对潮霉素B的抗性是由编码一种磷酸化潮霉素B的磷酸转移酶的基因赋予的,从而使潮霉素B失活。潮霉素B是一种有效的选择和维持细菌和真核细胞稳定转染载体含有大肠杆菌潮霉素B抗性基因(hyg或hph)。
当使用ClonaCell™-TCS培养基(目录#03814)时,该抗生素适用于选择耐潮霉素克隆的细胞培养,并适用于选择任何耐潮霉素转染的细胞类型。
Subtype
Supplements
Cell Type
CHO Cells, Hybridomas
Species
Mouse
Application
Cell Culture, Hybridoma Generation
Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Mouse |
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半固体甲基纤维素培养基,用于选择和克隆悬浮适应细胞和贴壁细胞(含血清)
化学定义,无动物成分,无血清,无蛋白质,无谷氨酰胺半固体甲基纤维素为基础的培养基,用于选择和克隆CHO细胞
化学定义,无动物成分,无血清,无蛋白质,无谷氨酰胺的液体培养基,用于培养CHO细胞
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