产品号 #74142_C
增强内皮细胞、上皮细胞、间充质细胞和少突胶质细胞的增殖和分化
总览
氢化可的松是一种具有抗炎和免疫抑制作用的糖皮质激素（Kang 等人；Langhoff 和 Ladefoged）。它也常用作内皮细胞、上皮细胞、间充质细胞或少突胶质细胞培养基的补充剂，因为它有助于细胞生长和分化。本产品以氢化可的松 21-半琥珀酸钠的形式提供，这是一种更易溶于水的氢化可的松形式。
在MyeloCult™ H5100 (Human；产品号 #05150) 和 MyeloCult™ M5300 (Mouse；产品号 #05350)，需要添加氢化可的松，用于长期培养和长期培养起始细胞 (LTC-IC) 检测。完整说明请参阅《人或小鼠 LTC-IC 检测技术手册》，该手册可在www.stemcell.com或联系我们索取副本。
维持和自我更新
· 增强外植体培养中角膜缘干细胞的增殖和自我更新 (Yu et al.)。
· 支持培养中未成熟人类髓系细胞的长期生长 (Salahuddin et al.)。
· 在胰岛素存在的情况下，增加培养小鼠乳腺上皮细胞中葡萄糖 6-磷酸脱氢酶的活性 (Oka & Perry)。
分化
· 与 ATRA、EGF、KGF 和 HGF 一起，促进脂肪来源干细胞的上皮分化 (Li et al.)。
· 促进小鼠胚胎干细胞 (ES) 来源的定形内胚层向肺泡上皮细胞分化 (Mokhber Dezfouli et al.)。
免疫学
· 抑制促炎细胞因子白介素 6 的生物活性（(IC₅₀ = 6.7 mM; Kang et al.)。
· 抑制体外植物血凝素 (PHA) 刺激的淋巴细胞的活性（Langhoff & Ladefoged）。
细胞类型
内皮细胞，淋巴细胞，乳腺细胞，间充质干/祖细胞，髓系细胞
种属
人，小鼠，非人灵长类，其它细胞系，大鼠
应用
分化，培养
研究领域
内皮细胞研究，上皮细胞研究，免疫
CAS 编号
125-04-2
化学式
C₂₅H₃₃O₈ • Na
纯度
≥98%
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
Publications (1)
Restraining Lysosomal Activity Preserves Hematopoietic Stem Cell Quiescence and Potency. Cell stem cell 2020
Abstract
Quiescence is a fundamental property that maintains hematopoietic stem cell (HSC) potency throughout life. Quiescent HSCs are thought to rely on glycolysis for their energy, but the overall metabolic properties of HSCs remain elusive. Using combined approaches, including single-cell RNA sequencing (RNA-seq), we show that mitochondrial membrane potential (MMP) distinguishes quiescent from cycling-primed HSCs. We found that primed, but not quiescent, HSCs relied readily on glycolysis. Notably, in vivo inhibition of glycolysis enhanced the competitive repopulation ability of primed HSCs. We further show that HSC quiescence is maintained by an abundance of large lysosomes. Repression of lysosomal activation in HSCs led to further enlargement of lysosomes while suppressing glucose uptake. This also induced increased lysosomal sequestration of mitochondria and enhanced the competitive repopulation ability of primed HSCs by over 90-fold in vivo. These findings show that restraining lysosomal activity preserves HSC quiescence and potency and may be therapeutically relevant.
更多信息
| Species | Human, Mouse, Non-Human Primate, Other, Rat |
|---|---|
| Cas Number | 125-04-2 |
| Chemical Formula | C₂₅H₃₃O₈ • Na |
| Purity | ≥ 98% |
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
