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Human Umbilical Vein Endothelial Cells

Frozen human umbilical vein endothelial cells, isolated and expanded in xeno-free conditions.

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产品优势

Obtain over 500,000 HUVECs from a single vial—guaranteed— isolated from a healthy donor

Achieve over 16 population doublings of HUVECs when cultured in xeno-free EC-Cult™-XF medium

Obtain HUVECs frozen at early passage 2, with over 90% viability prior to cryopreservation

Retain high expression of endothelial markers CD31 and CD144 through later passages with EC-Cult™-XF medium

Observe functional properties such as the formation of tubular networks and acetylated LDL uptake

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概述

实验数据

产品说明书及文档

应用领域

相关材料与文献

Streamline your assays with ready-to-use, ethically sourced, human umbilical vein endothelial cells (HUVECs). These cells are isolated from healthy single-donor umbilical cord veins (using non-invasive collagenase digestion) into EC-Cult™-XF medium and cryopreserved at passage 2 in animal component-free, cGMP-manufactured CryoStor® CS10 medium. Obtain a large number of HUVECs from a single vial for consistent results.

HUVECs are collected using Institutional Review Board (IRB)-approved consent forms and protocols. They can show expression of endothelial markers through later passages, an ability to form tubular networks, as well as uptake of acetylated LDL. HUVECs can be used as an endothelial cell model to study physiological and pathological pathways, such as inflammation, angiogenesis, oxidative stress, hypoxia, and blood vessel formation. With personalized service, custom products, and flexible delivery times, we help you get the cells you need.

Additional documentation and high-resolution HLA typing (Class I and Class II alleles) and CMV status is available upon request. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C. Full product information is provided on the Certificate of Analysis.

Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.

Data Figures

Phase contrast image of HUVECs cultured in EC-Cult™-XF shows how these cells grow as monolayers and retain their cobblestone-like morphology of endothelial cells.

Figure 1. HUVECs Cultured in EC-Cult™-XF Have Cobblestone-Like Morphology

Phase-contrast image of HUVECs cultured in EC-Cult™-XF. Cells grow as monolayers and retain their typical cobblestone-like morphology of endothelial cells.

Flow cytometry analysis shows that HUVECs cultured in EC-Cult™-XF medium retain the endothelial markers CD31 and CD144 through 19 passages better than those cultured in a commercial medium.

Figure 2. HUVECs Cultured Long-Term in Complete EC-Cult™-XF Retain Purity of Endothelial Markers.

HUVECs cultured and passaged in complete EC-Cult™-XF medium were compared to HUVECs cultured and passaged in an alternative commercial medium. Both sets of HUVECs were then analyzed by flow cytometry. Cells cultured in EC-Cult™-XF medium had higher retention of the endothelial markers CD31 and CD144 through 19 passages.

HUVECs can expand for higher passage numbers when cultured in EC-Cult™-XF medium when compared to alternative commercial media.

Figure 3. HUVECs Can Be Expanded For Greater Passage Numbers in EC-Cult™-XF Than in Alternative Commercial Media

HUVECs were cultured and expanded in EC-Cult™-XF medium or alternative commercial medium (A and B). HUVECs expanded in complete EC-Cult™-XF medium outperformed those in the alternative media, with population doublings continuing to increase beyond passage 16.

HUVECs, from passages 2 and 7, were plated in EC-Cult™-XF medium and grown for 24 hours. Cells were imaged at 2, 6, 18, and 24 hour time points, showing the ability to form defined tubular branching points as early as after 2 hours of plating.

Figure 4. HUVECs Form Tubular Networks When Cultured in Complete EC-Cult™-XF Medium

HUVECs, from passage 2 and passage 7, were plated at 10,000 cells/well (96-well plate) in complete EC-Cult™-XF medium and grown for 24 hours on Corning® Matrigel®. Cells were imaged at different time points and shown to form defined tubular branching points as early as after 2 hours of plating.

Labeled acetylated LDL was added to HUVECs at early (P2) and late (P7) passages. Cells were then imaged using a confocal microscope and shown to be functionally active and able to incorporate acetylated LDL at both P2 and P7.

Figure 5. HUVECs are Functionally Active at Early and Late Passages

The ability of HUVECs to metabolise acetylated LDL was examined by adding labeled acetylated LDL into the cells and incubated for 4-6 hours before imaging using a confocal microscope. Cells were shown to be functionally active and able to incorporate acetylated LDL at early (P2) and late (P7) passage when cultured in EC-Cult™-XF Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

Human Umbilical Vein Endothelial Cells (HUVECs)

Catalog #

200-0630

Lot #

All

Language

English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Hematopoietic Stem and Progenitor Cell Research

Cell Sourcing and Isolation

Expansion and Differentiation

Analysis