人脐静脉内皮细胞

在无异源条件下分离和扩增的冻存的人脐静脉内皮细胞

产品号 #(选择产品)

产品号 #200-0630_C

在无异源条件下分离和扩增的冻存的人脐静脉内皮细胞

产品优势

  • 单管含超过50万个HUVECs(保证从健康供体中分离)
  • 在无异源的EC-Cult™-XF培养基中培养,可实现HUVECs扩增倍数达到16倍以上
  • 获得第2代早期冻存的HUVECs,冻存前活率超过90%
  • 使用EC-Cult™-XF培养基,在后续传代中仍能保持高表达内皮细胞标志物CD31和CD144
  • 可观察管状网络形成和乙酰化LDL摄取等功能特性

总览

使用即用型、符合伦理的人脐静脉内皮细胞 (HUVECs) 简化您的检测流程。这些细胞从健康的单一供体脐带静脉中分离(采用非侵入性胶原酶消化法),使用EC-Cult™-XF培养基培养,并在第2代时冻存于无动物成分、符合cGMP标准的CryoStor® CS10冻存液中。可从单管中获得大量HUVECs以获得一致的结果。

HUVECs的采集遵循机构审查委员会 (IRB) 批准的知情同意书和方案。它们在后续传代中仍能表达内皮细胞标志物,具有形成管状网络的能力,并能摄取乙酰化LDL。HUVECs可作为内皮细胞模型,可用于研究生理和病理通路,如炎症、血管生成、氧化应激、缺氧和血管形成。我们提供个性化的服务、定制产品、灵活的交付周期,以确保您获得所需的细胞。

如有需要,我们可提供附加文档、高分辨率HLA分型(I类和II类等位基因)以及CMV状态。您可以在上方评论框中查看供体信息(例如BMI范围、吸烟状况、种族等)。供体将接受HIV-1、HIV-2、乙肝和丙肝筛查。完整的产品信息请参阅分析证书

某些产品只在特定地区出售。请通过info.cn@stemcell.com联系您的销售代表或技术支持以获取更多信息。

浏览有关原代细胞的常见问题解答

包含
CryoStor® CS10
 
细胞类型
HUVEC细胞(人脐静脉内皮细胞)
 
种属

 
细胞和组织来源
其它细胞系
 
研究领域
内皮细胞研究
 
供体状态
Normal
 
纯度
流式细胞术检测:CD31+CD144+ ≥ 95% 且 CD45+ ≤ 5%
 

Data Figures

Phase contrast image of HUVECs cultured in EC-Cult™-XF shows how these cells grow as monolayers and retain their cobblestone-like morphology of endothelial cells.

Figure 1. HUVECs Cultured in EC-Cult™-XF Have Cobblestone-Like Morphology

Phase-contrast image of HUVECs cultured in EC-Cult™-XF. Cells grow as monolayers and retain their typical cobblestone-like morphology of endothelial cells.

Flow cytometry analysis shows that HUVECs cultured in EC-Cult™-XF medium retain the endothelial markers CD31 and CD144 through 19 passages better than those cultured in a commercial medium.

Figure 2. HUVECs Cultured Long-Term in Complete EC-Cult™-XF Retain Purity of Endothelial Markers.

HUVECs cultured and passaged in complete EC-Cult™-XF medium were compared to HUVECs cultured and passaged in an alternative commercial medium. Both sets of HUVECs were then analyzed by flow cytometry. Cells cultured in EC-Cult™-XF medium had higher retention of the endothelial markers CD31 and CD144 through 19 passages.

HUVECs can expand for higher passage numbers when cultured in EC-Cult™-XF medium when compared to alternative commercial media.

Figure 3. HUVECs Can Be Expanded For Greater Passage Numbers in EC-Cult™-XF Than in Alternative Commercial Media

HUVECs were cultured and expanded in EC-Cult™-XF medium or alternative commercial medium (A and B). HUVECs expanded in complete EC-Cult™-XF medium outperformed those in the alternative media, with population doublings continuing to increase beyond passage 16.

HUVECs, from passages 2 and 7, were plated in EC-Cult™-XF medium and grown for 24 hours. Cells were imaged at 2, 6, 18, and 24 hour time points, showing the ability to form defined tubular branching points as early as after 2 hours of plating.

Figure 4. HUVECs Form Tubular Networks When Cultured in Complete EC-Cult™-XF Medium

HUVECs, from passage 2 and passage 7, were plated at 10,000 cells/well (96-well plate) in complete EC-Cult™-XF medium and grown for 24 hours on Corning® Matrigel®. Cells were imaged at different time points and shown to form defined tubular branching points as early as after 2 hours of plating.

Labeled acetylated LDL was added to HUVECs at early (P2) and late (P7) passages. Cells were then imaged using a confocal microscope and shown to be functionally active and able to incorporate acetylated LDL at both P2 and P7.

Figure 5. HUVECs are Functionally Active at Early and Late Passages

The ability of HUVECs to metabolise acetylated LDL was examined by adding labeled acetylated LDL into the cells and incubated for 4-6 hours before imaging using a confocal microscope. Cells were shown to be functionally active and able to incorporate acetylated LDL at early (P2) and late (P7) passage when cultured in EC-Cult™-XF Medium.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
200-0630
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (3)

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Species Human
Contains CryoStor® CS10
Purity ≥ 95% CD31+CD144+ and ≤ 5% CD45+ by flow cytometry
Cell And Tissue Source Other
Donor Status Normal
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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