产品号 #100-1299_C
骨硬化蛋白，His标签
总览
利用骨硬化蛋白调节骨重塑的动态平衡，可能通过Wnt/β-catenin信号级联，在体内和体外抑制骨形成(Ellies等；Lin等)。Sclerostin是cerberus/DAN糖蛋白家族的成员，其成员具有一个共享C端半胱氨酸结样（CTCK）结构域。这种破骨细胞来源的BMP拮抗剂在在骨和软骨中表达高度，同时也在肾脏和肝脏组织中表达（Weivoda等），它对BMP6和BMP7具有高亲和力，并对BMP2和BMP4具有较低亲和力（Kusu等人）。SOST基因突变与硬化症（Brunkow等）、van Buchem病（Staehling-Hampton等）和以骨密度增加为特征的骨发育不良疾病有关。该蛋白产物在多肽链的羧基端带有一个His标签。为确保各种应用中的一致性与可重复性，STEMCELL公司的硬化蛋白经过冻干，纯度≥87%，内毒素水平被验证为≤1.0 EU/μg蛋白。
亚型
细胞因子
别名
CDD，DAND6，SOST，SOST1，VBCH
种属
人
研究领域
代谢
纯度
≥ 87%
Data Figures
(A) The biological activity of Human Recombinant Sclerostin was tested by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The EC50 is defined as the effective concentration of the cytokine at which inhibition of enzyme production is at 50% of maximum. The EC50 in the above example is ≤ 1 μg/mL. (B) Human Recombinant Sclerostin was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant Sclerostin has a predicted molecular mass of 22.5 kDa but an apparent molecular mass of 29.9 kDa due to glycosylation.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant Sclerostin Catalog #
100-1299 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Sclerostin Catalog #
100-1299 Lot #
All Language
English Request Pricing
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更多信息
| Species | Human |
|---|---|
| Alternative Names | CDD, DAND6, SOST, SOST1, VBCH |
| Purity | ≥ 87% |
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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