产品号 #100-1299_C
Sclerostin, His tag
概述
Use sclerostin to alter bone remodeling homeostasis, where it inhibits bone formation in vivo and in vitro, likely through Wnt/β-catenin signaling cascades (Ellies et al.; Lin et al.). Sclerostin is a member of the cerberus/DAN family of glycoproteins whose members share a C-terminal cysteine-knot-like (CTCK) domain. Highly expressed in bone and cartilage, as well as in kidney, and liver tissues (Weivoda et al.), this osteoclast-derived BMP antagonist binds BMP6 and BMP7 with high affinity, and binds BMP2 and BMP4 with a lower affinity (Kusu et al.). Mutations in the SOST gene have been associated with sclerosteosis (Brunkow et al.), van Buchem disease (Staehling-Hampton et al.), and bone dysplasia disorders, characterized by increased bone density. This protein product contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, sclerostin from STEMCELL comes lyophilized with ≥ 87% purity, and endotoxin levels are verified to be ≤ 1.0 EU/μg protein.
Subtype
Cytokines
Alternative Names
CDD, DAND6, SOST, SOST1, VBCH
Species
Human
Area of Interest
Metabolism
Purity
≥ 87%
Data Figures
(A) The biological activity of Human Recombinant Sclerostin was tested by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The EC50 is defined as the effective concentration of the cytokine at which inhibition of enzyme production is at 50% of maximum. The EC50 in the above example is ≤ 1 μg/mL. (B) Human Recombinant Sclerostin was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant Sclerostin has a predicted molecular mass of 22.5 kDa but an apparent molecular mass of 29.9 kDa due to glycosylation.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet Product Name
Human Recombinant Sclerostin Catalog #
100-1299 Lot #
All Language
English Document Type
Safety Data Sheet Product Name
Human Recombinant Sclerostin Catalog #
100-1299 Lot #
All Language
English Request Pricing
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