产品号 #100-2073_C
Gremlin-1，不含动物成分
Gremlin-1是一种分泌蛋白，特异性结合骨形态发生蛋白（BMP）家族蛋白，包括BMP-2和BMP-4，阻止其与其受体相互作用（Hsu等）。Gremlin-1在结肠基底隐窝中表达，它被认为有助于干细胞微环境的维持（Kosinski等）。Gremlin-1的表达水平在疾病中升高，包括一些结肠肿瘤（Kosinski等）、肝脏疾病和糖尿病（Baboota等）。此外，Gremlin-1的过表达已被证明以不依赖于VEGFR2和BMP的方式促进癌细胞的迁移、增殖和侵袭性（Kim等）。Gremlin-1 也被用于培养人结肠上皮组织和癌症类器官（Urbischek 等人）。本产品不含动物成分（ACF）。
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human Recombinant Gremlin-1, ACF
(A) The biological activity of Human Recombinant Gremlin-1, ACF was tested by its ability to inhibit BMP-2 activity in stably transfected HEK293T cells using a luciferase reporter assay. Firefly luciferase activity is normalized to the control Renilla luciferase activity.The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.88 nM (34 ng/mL).
(B) 7 μg of Human Recombinant Gremlin-1, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Gremlin-1, ACF has a predicted molecular mass of 18 kDa (monomer). The characteristic diffused bands are due to the dissociation of the non-covalent dimer during electrophoresis.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Human Recombinant Gremlin-1, ACF Catalog #
100-2073 Lot #
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更多信息
| Species | Human, Other |
|---|---|
| Formulation Category | Animal Component-Free |
| Alternative Names | BMP antagonist 1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, DRM |
| Purity | ≥ 98% by SDS-PAGE |
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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