产品号 #100-2075_C
生长分化因子15，不含动物成分
生长分化因子-15 (GDF-15)，又称巨噬细胞抑制因子-1 (MIC-1)，是转化生长因子β (TGF-β)超家族的成员。在人体细胞组织中，生理性的GDF-15表达水平较低或不存在，但GDF-15水平升高与细胞应激、炎症、代谢疾病、癌症和其他疾病有关(Emmerson等；Wischhusen等)。GDF-15通过GFRAL （GDNF Family Receptor Alpha Like）及其共受体RET进行信号转导，导致RET的磷酸化，并激活ERK和AKT信号通路（Emmerson 等）。人重组GDF-15已用于小鼠研究（Kim等）。该产品经检测TGF-β活性为阴性，TGF-β是商业来源GDF-15的常见污染物（Olsen等）。本产品不含动物成分（ACF）。
Data Figures
Figure 1. Biological Activity and Molecular Mass of Human Recombinant GDF-15, ACF
(A) Human Recombinant GDF-15, ACF was tested by its ability to activate SMAD2/3 in HEK293T cells using a luciferase reporter assay. Firefly luciferase activity is normalized to the control Renilla luciferase activity. Increasing concentration of Human Recombinant GDF-15 did not result in increasing SMAD2/3 activity, indicating it is free of contamination with TGF-β or related growth factors.
(B) 7 μg of Human Recombinant GDF-15, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant GDF-15, ACF has a predicted molecular mass of 25 kDa (dimer).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Human Recombinant GDF-15, ACF Catalog #
100-2076 Lot #
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Human Recombinant GDF-15, ACF Catalog #
100-2076 Lot #
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更多信息
| Species | Human |
|---|---|
| Formulation Category | Animal Component-Free |
| Alternative Names | Macrophage inhibitory cytokine 1 (MIC-1), NSAID-activated gene 1 protein (NAG-1), NSAID-regulated gene 1 protein (NRG-1) |
| Purity | ≥ 98% by SDS-PAGE |
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