• Reportable range: 0.32 - 32 pM. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. Recombinant human LAP (latency associated protein) homodimer is the analyte standard of this ELISA. Determination of latent TGF-β1 using the standard curve is based on a molar comparison. 1 pM LAP (54 pg/mL) corresponds to 1 pM latent TGF-β1 (80 pg/mL). • Sensitivity: The limit of detection of this assay is 0.13 pM. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Recovery: A mid-curve recovery of 100% was determined by dilution of plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 3.0% (CV). The inter-assay precision of this assay is 10.4% (CV).
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