The Human Latent Transforming Growth Factor-beta 1 (TGF-β1) Kit is designed for the quantitative detection and measurement of latent human TGF-β1 in biological fluids such as plasma and cell culture supernatants. TGF-β1 is produced primarily by platelets and bone, and in smaller amounts by most nucleated cells and tumors. TGF-β1 stimulates cells of mesenchymal origin, but inhibits cells of epithelial or neuroectodermal origin. TGF-β1 associates with Latency Associated Protein (LAP) to form a latent complex. This kit detects the LAP entity of the complex.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Subtype Complete Kits
Cell Type Dendritic Cells, Macrophages, Platelets, T Cells, Tumor Cells
Species Human
Area of Interest Immunology
Data Figures
Representative Standard Curve
• Reportable range: 0.32 - 32 pM. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. Recombinant human LAP (latency associated protein) homodimer is the analyte standard of this ELISA. Determination of latent TGF-β1 using the standard curve is based on a molar comparison. 1 pM LAP (54 pg/mL) corresponds to 1 pM latent TGF-β1 (80 pg/mL). • Sensitivity: The limit of detection of this assay is 0.13 pM. This is the analyte concentration with absorbance two standard deviations higher than the zero standard. • Recovery: A mid-curve recovery of 100% was determined by dilution of plasma samples in repeated experiments. • Precision: The intra-assay precision of this assay is 3.0% (CV). The inter-assay precision of this assay is 10.4% (CV).
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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