The Human Immunoglobulin E (IgE) ELISA Kit is designed for the quantitative detection and measurement of human IgE in biological fluids such as serum, plasma, and cell culture supernatants. IgE is predominantly found in the lungs, skin, and mucous membranes and is the least common immunoglobulin in serum. IgE plays a fundamental role in allergic reactions by stimulating degranulation of eosinophils, mast cells, and basophils through interaction with the Fc epsilon Receptor I (FcεRI).
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the immunoglobulin. The captured immunoglobulin is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase (SA-HRP), which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of immunoglobulin in the sample. The concentration of the immunoglobulin is determined by comparison to a serial dilution of the immunoglobulin standard analyzed in parallel.
Data Figures
Representative Standard Curve
• Reportable Range: 0.1 - 32 ng/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 0.069 ng/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: The analyte standard of this ELISA was calibrated against NIBSC international standard 75/502.
• Recovery: A mid-curve recovery of 80 - 86% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 2.8% (CV). The inter-assay precision of this assay is 5.5% (CV).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human IgE ELISA Kit
Catalog #
02033
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 10
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Document Type
Safety Data Sheet 11
Product Name
Human IgE ELISA Kit
Catalog #
02033, 02032
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Resources and Publications
Educational Materials (2)
Request Pricing
Thank you for your interest in IntestiCult™ Organoid Growth Medium (Human). Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our
privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
欢迎您关注STEMCELL Technologies的微信公众平台
扫描二维码或搜索微信号STEMCELLTech，即可关注我们的微信平台，第一时间接收丰富的技术资源和最新的活动信息。
如您有任何问题，欢迎发消息给STEMCELLTech微信公众平台，或与我们通过电话/邮件联系：400 885 9050 INFO.CN@STEMCELL.COM。