人ACE2酶联免疫吸附测定试剂盒

用于检测和测量人体ACE2

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产品号 #100-0687_C

用于检测和测量人体ACE2

产品组分包括

人ACE2酶联免疫吸附测定试剂盒（目录#100-0XXX）

人ACE2酶联免疫吸附试验板
人用ACE2标准品，2瓶
Human ACE2 Biotinylated Detection Antibody, 120µL
Sa-hrp, 120µl
人ACE2抗体稀释液，15ml
SA-HRP稀释液，15ml
ELISA稀释液，50 mL
洗涤缓冲液（25X）， 20ml
TMB底物，10ml
停止溶液，10ml
粘接板盖，4个

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概述

人血管紧张素转换酶2 (ACE2) ELISA试剂盒是专为定量检测和测量人血管紧张素转换酶2 （ACE2）在生物流体，如血清，血浆和细胞培养上清。

ACE2是一种I型整体膜蛋白。它在毛细血管丰富的器官如肺、心脏、血管、肾脏、肝脏和胃肠道中高度表达。ACE2受体是SARS-CoV-2刺突(S)糖蛋白的靶标。这种相互作用允许病毒进入宿主细胞，使ACE2成为抗病毒治疗的潜在靶点。

该检测基于夹心ELISA方法，将样品添加到预先包被捕获的ACE2特异性抗体的ELISA条带板上。捕获的ACE2通过添加生物素化检测抗体检测，然后添加链霉亲和素-辣根过氧化物酶，该酶与生物素化抗体结合。添加显色酶底物3,3 ‘,5,5 ’四甲基联苯胺（TMB）可得到着色产物，其强度与样品中ACE2的浓度成正比。ACE2的浓度是通过与平行分析的标准品的连续稀释度进行比较来确定的。

Data Figures

Representative standard curve for Human ACE2 ELISA Kit

Figure 1. Representative Standard Curve

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 0.156 - 10 ng/mL. The lower limit of detection (LLD) of this assay is 39 pg/mL. This is the lowest protein concentration that could be differentiated from zero as determined by the mean OD value of 20 replicates of the zero standard added by their three standard deviations. The intra- and inter-assay precision (CV) of this assay are < 8% and < 10%, respectively. This assay has high specificity for the detection of human ACE2. No significant cross-reactivity or interference is known to occur between human ACE2 and analogues.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Product Information Sheet

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Human ACE2 ELISA Kit

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