Antibiotic G418 is an aminoglycoside related to gentamicin and is toxic to bacteria, yeast, higher plants, mammalian cells, protozoans, and helminths. G418 binds to 80S ribosomes in prokaryotic and eukaryotic cells and inhibits protein synthesis, resulting in cell death. Resistance to the antibiotic is conferred by aminoglycoside-3-phosphotransferase. Vectors containing the G418 resistance gene (neo) are commonly used for transfecting eukaryotic cells, with selection of transfected cells achieved by culturing them in the presence of G418. Cells may exhibit variable resistance to the antibiotic and can take up to a week to die (adherent cells may be more sensitive).
The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), ClonaCell™-CHO CD Medium (Catalog #03815), ClonaCell™-CHO ACF Medium (Catalog #03816), ClonaCell™CHO CD Liquid Medium (Catalog #03817), or other cell culture media.
The G418 antibiotic is suitable for use in cell culture for the selection and maintenance of G418-resistant clones when using ClonaCell™-TCS Medium (Catalog #03814), ClonaCell™-CHO CD Medium (Catalog #03815), ClonaCell™-CHO ACF Medium (Catalog #03816), ClonaCell™CHO CD Liquid Medium (Catalog #03817), or other cell culture media.
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Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
G418
Catalog #
03812
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Workflow Stages
Workflow Stages for CHO and Other Cell Line Development
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Educational Materials (1)
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