产品号 #03812_C
用于选择转染的哺乳动物细胞的抗生素
抗生素G418是一种与庆大霉素相关的氨基糖苷,对细菌、酵母、高等植物、哺乳动物细胞、原生动物和蠕虫有毒性。G418与原核和真核细胞中的80S核糖体结合,抑制蛋白质合成,导致细胞死亡。对抗生素的耐药性是由氨基糖苷-3-磷酸转移酶赋予的。含有G418抗性基因(neo)的载体通常用于转染真核细胞,通过在G418存在下培养来实现转染细胞的选择。细胞可能对抗生素表现出不同的耐药性,并可能需要长达一周的时间才能死亡(贴壁细胞可能更敏感)。
G418抗生素适用于使用ClonaCell™-TCS培养基(目录#03814),ClonaCell™-CHO CD培养基(目录#03815),ClonaCell™-CHO ACF培养基(目录#03816),ClonaCell™-CHO CD液体培养基(目录#03817)或其他细胞培养基时,用于选择和维持G418抗性克隆的细胞培养。
Cell Type
CHO Cells, Hybridomas
Species
Mouse
Application
Hybridoma Generation, Toxicity Assay
Area of Interest
Cell Line Development, Drug Discovery and Toxicity Testing, Hybridoma Generation
CAS Number
108321-42-2
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Species | Mouse |
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Cas Number | 108321-42-2 |
半固体甲基纤维素培养基,用于选择和克隆悬浮适应细胞和贴壁细胞(含血清)
化学定义,无动物成分,无血清,无蛋白质,无谷氨酰胺半固体甲基纤维素为基础的培养基,用于选择和克隆CHO细胞
化学定义,无动物成分,无血清,无蛋白质,无谷氨酰胺的液体培养基,用于培养CHO细胞
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