Use Falcon® Round-Bottom Polypropylene Tubes, 5 mL for a wide variety of sample preparation, storage, and centrifugation tasks. These sterile, high-quality, and RNase-/DNase-free tubes are required for select cell isolation, flow cytometry, and fluorescence-activated cell sorting (FACS) protocols. The Falcon® Round-Bottom Polypropylene Tubes, 5 mL are available with caps in packs of 25 or 500 tubes, and without caps in packs of 125 or 1000 tubes.
Subtype Tubes
Species Human, Mouse, Non-Human Primate, Other, Rat
FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver. T. W. Braun et al. STAR protocols 2022 mar
Abstract
Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24low progenitors are less mature and have greater proliferative capacity than CD71/CD24high. We present protocols for isolation of CD71/CD24low progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24low progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).
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