The EasySep™ Non-Human Primate B Cell Isolation Kit is designed to isolate untouched and highly purified B cells from fresh or previously frozen non-human primate peripheral blood mononuclear cells (PBMCs) in as little as 20 minutes by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
This kit has been verified for use with rhesus and cynomolgus macaques.
This kit has been verified for use with rhesus and cynomolgus macaques.
Data Figures
Figure 1. EasySep™ Non-Human Primate B Cell Isolation Kit
Starting with rhesus PBMCs, the B cell content (CD20+) of the isolated fraction is typically 91.4 ± 5.2% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 10.4% and 94.8%, respectively.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0345
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Non-Human Primate B Cell Isolation Kit
Catalog #
100-0347
Lot #
All
Language
English
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for B Cell Research
Resources and Publications
Educational Materials (4)
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