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EasySep™ 小鼠F4/80正选试剂盒

从小鼠脾细胞、肺组织和腹腔灌洗液中通过免疫磁珠正选小鼠F4/80+细胞

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产品号 #100-0659

从小鼠脾细胞、肺组织和腹腔灌洗液中通过免疫磁珠正选小鼠F4/80+细胞

产品优势

  • 操作简单、快捷，且无需分离柱
  • 纯度高达90%
  • 分选得到的细胞无荧光标记

产品组分包括

  • EasySep™小鼠F4/80正选抗体混合物组分A， 0.3 mL
  • EasySep™小鼠F4/80正选抗体混合物组分B， 0.6 mL
  • EasySep™ Dextran RapidSpheres™ 50100 磁珠，2 x 1 mL
  • 小鼠FcR多克隆阻断剂（产品号 #300-0902），1.2 mL
main product photo
EasySep™ 小鼠F4/80正选试剂盒
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

使用EasySep™小鼠F4/80正选试剂盒，通过免疫磁珠正选技术，可轻松从小鼠脾细胞、肺组织和腹腔灌洗液样本中分离高纯度小鼠F4/80+细胞。EasySep™技术结合单克隆抗体的特异性和无柱磁分选系统的简便性，已在发表的研究中广泛应用超过20年。

在此EasySep™正选流程中，目标细胞通过识别F4/80的抗体复合物和磁珠进行标记，并通过EasySep™磁极分选。最后只需简单倾倒或弃去非目的细胞，目的细胞则保留在试管中。磁珠分选最快仅需24分钟，所需的F4/80+细胞可立即用于下游应用，例如流式细胞术、培养和基于细胞的实验。
了解更多关于免疫磁珠EasySep™技术的工作原理。探索更多为您实验流程优化的其它产品，包括培养基、补充剂、抗体等。

MAGNET COMPATIBILITY
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
 
SUBTYPE
Cell Isolation Kits
 
CELL TYPE
Macrophages
 
SPECIES
Mouse
 
SAMPLE SOURCE
Lung, Other, Spleen
 
SELECTION METHOD
Positive
 
APPLICATION
Cell Isolation
 
BRAND
EasySep
 
AREA OF INTEREST
Immunology, Infectious Diseases

实验数据

FACS plot showing cell populations from lung tissue pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 1. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Lung Tissue

Starting with a naïve mouse lung single-cell suspension, the F4/80+ cell content of the isolated fraction is typically 94.3 ± 2.8% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 26.3% and 95.3%, respectively.

Typical Profile for F4/80+ Cells Isolated from Peritoneal Lavage Using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 2. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Peritoneal Lavage

Starting with lavage cells from the peritoneal cavity of a naïve mouse, the F4/80+ cell content of the isolated fraction is typically 97.0 ± 0.4% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 47.9% and 97.0%, respectively.

FACS plot showing cell populations from spleen pre- and post-cell isolation using the EasySep™ Mouse F4/80 Positive Selection Kit

Figure 3. Typical EasySep™ Mouse F4/80 Positive Cell Isolation Profile from Spleen

Starting with naïve mouse splenocytes, the F4/80+ cell content of the isolated fraction is typically 88.8 ± 3.4% (mean ± SD) using the purple EasySep™ magnet. In the above example, the purities of the start and final isolated fractions are 5.4% and 90.6%, respectively.

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产品说明书及文档

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet - Spleen
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158053 or lower
Language
English
Document Type
Product Information Sheet - Peritoneal Lavage
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Lung Tissue
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158053 or lower
Language
English
Document Type
Product Information Sheet - Spleen
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Product Information Sheet - Peritoneal Lavage
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158053 or lower
Language
English
Document Type
Product Information Sheet - Lung Tissue
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
1000158054 or higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
EasySep™ Mouse F4/80 Positive Selection Kit
Catalog #
100-0659
Lot #
All
Language
English
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应用领域

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
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