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EasySep™小鼠生物素正选试剂盒II

免疫磁珠正选试剂盒
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产品号 #（选择产品）

产品号 #17665_C

免疫磁珠正选试剂盒

产品优势

  • 快速、简单
  • 无需分离柱

产品组分包括

  • EasySep™小鼠生物素 正选试剂盒II（产品号 #17665）
    • EasySep™生物素分选抗体混合物，1 mL
    • 小鼠FcR阻断剂，0.1 mL    
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 一抗偶联物专用管（手动操作无需使用），1支
  • RoboSep™小鼠生物素正选试剂盒II（产品号 #17665RF）
    • EasySep™生物素分选抗体混合物，1 mL
    • 小鼠FcR阻断剂，0.1 mL    
    • EasySep™ Dextran RapidSpheres™磁珠，1 mL
    • RoboSep™ 一抗偶联物专用管（手动操作无需使用），1支
    • RoboSep™ 缓冲液（产品号 #20104）
    • RoboSep™过滤枪 头（产品号#20125）x 2
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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产品说明书及文档
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总览

EasySep™ 小鼠生物素正选试剂盒 II 专为分选 经生物素化抗体标记的细胞而设计。目的细胞被生物素化抗体复合物和磁珠标记，使用EasySep™磁极分选被标记的细胞，无需使用分离柱。目的细胞被保留在试管中，而非目的细胞被倾倒出。

本产品替代 EasySep™ 小鼠生物素正选试剂盒（产品号 #18556）。

如需高纯度且不带磁珠正选细胞，推荐使用 EasySep™ Release 小鼠生物素正选试剂盒（产品号 #17655）。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
 
亚型
细胞分选试剂盒
 
细胞类型
B 细胞，树突状细胞（DCs），粒细胞及其亚群，造血干/祖细胞，巨噬细胞，骨髓基质细胞，间充质干/祖细胞，单核细胞，单个核细胞，髓系细胞，NK 细胞，其它细胞系，血浆，T 细胞
 
种属
小鼠
 
样本来源
Bone Marrow，其它细胞系，Spleen
 
筛选方法
Positive
 
应用
细胞分选
 
品牌
EasySep，RoboSep
 
研究领域
免疫
 

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Data Figures

Starting with mouse splenocytes, the purities of the start and final isolated fractions in the above example are 20.5% and 97.5%, respectively, using a biotinylated anti-mouse CD4 antibody and EasySep™ Mouse Biotin Positive Selection Kit II (as assessed using PE-conjugated streptavidin).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
RoboSep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
EasySep™ Mouse Biotin Positive Selection Kit II
Catalog #
17665
Lot #
All
Language
English

Resources and Publications

Educational Materials (7)

EasySep™ Cell Separation Technology
Brochure
EasySep™ Cell Separation Technology
Tools for Your Immunology Research
Brochure
Tools for Your Immunology Research
Endothelial Protein C Receptor (EPCR): A New Marker for Identification and Positive Selection of Mouse Hematopoietic Stem Cells
Technical Bulletin
Endothelial Protein C Receptor (EPCR): A New Marker for Identification and Positive Selection of Mou...
Frequencies and Percentages of Mouse Immune Cell Types
Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Human Immune Cytokines
Wallchart
Human Immune Cytokines
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
1:13
Video
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation
1:57
Video
How EasySep™ Magnetic Cell Separation Technology Works: Fast and Easy Cell Isolation

Publications (1)

Blocking CCL8-CCR8-Mediated Early Allograft Inflammation Improves Kidney Transplant Function. A. Dangi et al. Journal of the American Society of Nephrology : JASN 2022 oct

Abstract

BACKGROUND In kidney transplantation, early allograft inflammation impairs long-term allograft function. However, precise mediators of early kidney allograft inflammation are unclear, making it challenging to design therapeutic interventions. METHODS We used an allogeneic murine kidney transplant model in which CD45.2 BALB/c kidneys were transplanted to CD45.1 C57BL/6 recipients. RESULTS Donor kidney resident macrophages within the allograft expanded rapidly in the first 3 days. During this period, they were also induced to express a high level of Ccl8, which, in turn, promoted recipient monocyte graft infiltration, their differentiation to resident macrophages, and subsequent expression of Ccl8. Enhanced graft infiltration of recipient CCR8+ T cells followed, including CD4, CD8, and ?? T cells. Consequently, blocking CCL8-CCR8 or depleting donor kidney resident macrophages significantly inhibits early allograft immune cell infiltration and promotes superior short-term allograft function. CONCLUSIONS Targeting the CCL8-CCR8 axis is a promising measure to reduce early kidney allograft inflammation.
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更多信息

更多信息
Molecular Weight 1647,1648,1649
Species Mouse
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Sample Source Bone Marrow, Other, Spleen
Selection Method Positive
Target • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • EasyEights™ EasySep™ Magnet (Catalog #18103) • RoboSep™-S (Catalog #21000)
Pathway Positive
Legal Statement: Users of this kit should ensure that they are entitled to use the antibody of interest. STEMCELL Technologies Inc. is not responsible for patent infringements or violations that may occur when using this product. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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ISO 13485 和 ISO 9001 质量管理体系认证

ISO 14001 环境管理体系认证

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