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EasySep™人TCR Alpha/Beta去除试剂盒

人TCRαβ+细胞的免疫磁珠去除
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产品号 #（选择产品）

产品号 #17847_C

人TCRαβ+细胞的免疫磁珠去除

产品优势

  • 操作简单、快捷，且无需分离柱
  • 分选得到的细胞不带标记

产品组分包括

  • EasySep™人TCR Alpha/Beta去除试剂盒（产品号#17847）
    • EasySep™人 TCR Alpha/Beta去除抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™ 磁珠，2 x 1mL
  • EasySep™人TCR Alpha/Beta去除试剂盒（产品号#100-1660）
    • EasySep™人TCR Alpha/Beta去除抗体混合物，10 mL
    • EasySep™ Dextran RapidSpheres™磁珠，2 x 10 mL
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总览
实验数据
产品说明书及文档
应用领域
相关材料与文献
相关产品

总览

使用 EasySep™ 人 TCR Alpha/Beta 去除试剂盒，通过免疫磁珠正选技术，高效去除白细胞单采术样本或扩增的 TCRαβ 基因敲除细胞培养样本中的人 T 细胞受体 α/β 阳性（TCRαβ⁺）细胞。

EasySep™ 技术结合了单克隆抗体的特异性和无柱磁选系统的简便性，在过去 20 多年中被广泛应用于发表的科学研究中。

这种简便、优化的 EasySep™ 操作流程包括使用识别 TCRαβ 的抗体复合物和磁珠对细胞进行标记。标记后的细胞通过 EasySep™磁极，仅需倾倒或移液吸出未标记细胞，即可完成分选操作。TCRαβ⁺ 细胞会保留在管中。整个细胞分选过程最快仅需 13 分钟，所得目的细胞可直接用于流式细胞术、细胞培养或冻存等下游应用。

如需从白细胞单采术样本中大规模去除人 T 细胞受体α/β阳性（TCRαß+）细胞，请参阅大规格（1x10^10细胞）试剂盒（产品号#100-1660）。

了解更多关于 EasySep™ 免疫磁珠技术的工作原理。探索更多可优化您的实验流程的相关产品，包括培养基、添加物、抗体等。

磁体兼容性
• EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • Easy 250 EasySep™ Magnet (Catalog #100-0821)
 
亚型
细胞分选试剂盒
 
细胞类型
T 细胞
 
种属
人
 
样本来源
Leukapheresis，其它细胞系
 
筛选方法
删除
 
应用
细胞分选
 
品牌
EasySep
 
研究领域
免疫，细胞治疗开发
 

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Data Figures

The content of TCRab+ cells before and after the cell isolation procedure are 19.1% and 0.1%, respectively.

Figure 1. Typical EasySep™ Human TCR Alpha/Beta Depletion Profile

In the above example, the frequencies of CD3+TCRαß+ cells in the starting and depleted fractions are 19.1% and 0.1%, respectively.

Residual TCRαβ+ Cells Can Be Removed from Expanded TRAC Knockout Cell Populations Using EasySep™ Human TCRαβ Depletion Kit

Figure 2. EasySep™ Human TCRαβ Depletion Kit Can Be Used to Remove Residual TCRαβ+ Cells from Expanded TRAC Knockout Cell Populations After Genome Editing of Human Primary T Cells Using CRISPR-Cas9

Gene-edited TCRαβ knockout T cells were expanded using ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 - 10 days, harvested, and resuspended in EasySep™ Buffer (Catalog # 20144) at 5 x 10⁷ cells/mL. Following a 13-minute EasySep™ depletion protocol using EasySep™ Human TCRαβ Depletion Kit (Catalog #17847), flow cytometry was performed to assess the depletion of TCRαβ+ cells. Representative data plots are shown for the frequency of TCRαβ cells in the expanded TRAC knockout cell population before and after EasySep™ depletion.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
100-1660
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
17847
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
100-1660
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
17847
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
17847
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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更多信息
Species Human
Magnet Compatibility • EasySep™ Magnet (Catalog #18000) • “The Big Easy” EasySep™ Magnet (Catalog #18001) • Easy 50 EasySep™ Magnet (Catalog #18002) • Easy 250 EasySep™ Magnet (Catalog #100-0821)
Sample Source Leukapheresis, Other
Selection Method Depletion
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
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