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EasySep™人TCR Alpha/Beta去除试剂盒

人TCRαβ+细胞的免疫磁珠去除

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产品号 #17847

人CD271+细胞的免疫磁珠正选

产品优势

  • 快速、易于操作，且无需分离柱
  • 获得的活细胞无标记

产品组分包括

  • EasySep™人TCR Alpha/Beta去除试剂盒（产品号#17847）
    • EasySep™人TCR Alpha/Beta去除抗体混合物，1 mL
    • EasySep™ Dextran RapidSpheres™ 50101 磁珠，2 x 1mL
    • EasySep™人TCR Alpha/Beta去除试剂盒（产品号#100-1660）
      • EasySep™人TCR Alpha/Beta去除抗体混合物，10 mL
      • EasySep™ Dextran RapidSpheres™磁珠，2 x 10 mL
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EasySep™人TCR Alpha/Beta去除试剂盒
概述
实验数据
产品说明书及文档
应用领域
相关材料与文献

概述

使用 EasySep™ 人 TCR Alpha/Beta 去除试剂盒，通过免疫磁性正选技术，高效去除白细胞分离术样本或扩增的 TCRαβ 基因敲除细胞培养样本中的人 T 细胞受体 α/β 阳性（TCRαβ⁺）细胞。
EasySep™ 技术结合了单克隆抗体的特异性和无柱磁选系统的简便性，在过去 20 多年中被广泛应用于发表的科学研究中。
这种简便、优化的 EasySep™ 操作流程包括使用识别 TCRαβ 的抗体复合物和磁珠对细胞进行标记。标记后的细胞通过 EasySep™磁力架与未标记细胞分离，仅需倾倒或移液取出未标记细胞，即可完成分离。TCRαβ⁺ 细胞会保留在管中。整个磁性细胞分选过程最快仅需 13 分钟，所得目标细胞可直接用于流式细胞术、细胞培养或冷冻保存等下游应用。
如需从白细胞分离术样本中大规模去除人T细胞受体α/β+阳性（TCRαß+）细胞，请参阅大规格（1x10^10细胞）试剂盒（产品号#100-1660）。
了解更多关于 EasySep™ 免疫磁性技术的工作原理。探索更多可优化您的实验流程的相关产品，包括培养基、添加物、抗体等。

MAGNET COMPATIBILITY
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• Easy 250 EasySep™ Magnet (Catalog #100-0821)
 
SUBTYPE
Cell Isolation Kits
 
CELL TYPE
T Cells
 
SPECIES
Human
 
SAMPLE SOURCE
Leukapheresis, Other
 
SELECTION METHOD
Depletion
 
APPLICATION
Cell Isolation
 
BRAND
EasySep
 
AREA OF INTEREST
Immunology, Cell Therapy Developmen

实验数据

The content of TCRab+ cells before and after the cell isolation procedure are 19.1% and 0.1%, respectively.

Figure 1. Typical EasySep™ Human TCR Alpha/Beta Depletion Profile

In the above example, the frequencies of CD3+TCRαß+ cells in the starting and depleted fractions are 19.1% and 0.1%, respectively.

Residual TCRαβ+ Cells Can Be Removed from Expanded TRAC Knockout Cell Populations Using EasySep™ Human TCRαβ Depletion Kit

Figure 2. EasySep™ Human TCRαβ Depletion Kit Can Be Used to Remove Residual TCRαβ+ Cells from Expanded TRAC Knockout Cell Populations After Genome Editing of Human Primary T Cells Using CRISPR-Cas9

Gene-edited TCRαβ knockout T cells were expanded using ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981) for 7 - 10 days, harvested, and resuspended in EasySep™ Buffer (Catalog # 20144) at 5 x 10⁷ cells/mL. Following a 13-minute EasySep™ depletion protocol using EasySep™ Human TCRαβ Depletion Kit (Catalog #17847), flow cytometry was performed to assess the depletion of TCRαβ+ cells. Representative data plots are shown for the frequency of TCRαβ cells in the expanded TRAC knockout cell population before and after EasySep™ depletion.

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产品说明书及文档

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
100-1660
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
17847
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
100-1660
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
17847
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human TCR Alpha/Beta Depletion Kit
Catalog #
17847
Lot #
All
Language
English

应用领域

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
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